Journal article
Bioaugmenting the poplar rhizosphere to enhance treatment of 1,4-dioxane
The Science of the total environment, Vol.744, pp.140823-140823
11/20/2020
DOI: 10.1016/j.scitotenv.2020.140823
PMID: 32821670
Abstract
1,4-Dioxane is a highly mobile and persistent groundwater pollutant that often forms large dilute plumes. Because of this, utilizing aggressive pump-and-treat and ex-situ technologies such as advanced oxidation can be prohibitively expensive. In this study, we bioaugmented the poplar rhizosphere with dioxane-degrading bacteria Mycobacterium dioxanotrophicus PH-06 or Pseudonocardia dioxanivorans CB1190 to enhance treatment of 1,4-dioxane in bench-scale experiments. All treatments tested removed 10 mg/L dioxane to near health advisory levels (<4 μg/L). However, PH-06-bioaugmented poplar significantly outperformed all other treatments, reaching <4 μg/L in only 13 days. Growth curve experiments confirmed that PH-06 could not utilize root extract as an auxiliary carbon source for growth. Despite this limitation, our findings suggest that PH-06 is a strong bioaugmentation candidate to enhance the treatment of dioxane by phytoremediation. In addition, we confirmed that CB1190 could utilize both 1,4-dioxane and root extract as substrates. Finally, we demonstrated the large-scale production of these two strains for use in the field. Overall, this study shows that combining phytoremediation and bioaugmentation is an attractive strategy to treat dioxane-contaminated groundwater to low risk-based concentrations (~1 μg/L).
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•1,4-Dioxane is a mobile groundwater pollutant that threatens human health.•Phytoremediation with poplar removed 1,4-dioxane to low concentrations (~1 μg/L).•Adding dioxane-degrading microorganisms accelerated dioxane removal by poplar.•Select dioxane-degraders can utilize poplar root extract as an auxiliary substrate.•Dioxane-degraders were grown in fermenters for field-scale implementation.
Details
- Title: Subtitle
- Bioaugmenting the poplar rhizosphere to enhance treatment of 1,4-dioxane
- Creators
- Reid Simmer - Department of Civil and Environmental Engineering, College of Engineering, The University of Iowa, Iowa City, IA, USAJacques Mathieu - Department of Civil and Environmental Engineering, College of Engineering, Rice University, Houston, TX, USAMarcio L.B da Silva - Department of Civil and Environmental Engineering, College of Engineering, Rice University, Houston, TX, USAPhilip Lashmit - Center for Biocatalysis and Bioprocessing, Office for the Vice President for Research and Economic Development, University of Iowa Research Park, The University of Iowa, Coralville, IA, USASridhar Gopishetty - Center for Biocatalysis and Bioprocessing, Office for the Vice President for Research and Economic Development, University of Iowa Research Park, The University of Iowa, Coralville, IA, USAPedro J.J Alvarez - Department of Civil and Environmental Engineering, College of Engineering, Rice University, Houston, TX, USAJerald L Schnoor - Department of Civil and Environmental Engineering, College of Engineering, The University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- The Science of the total environment, Vol.744, pp.140823-140823
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.scitotenv.2020.140823
- PMID
- 32821670
- ISSN
- 0048-9697
- eISSN
- 1879-1026
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation; DOI: 10.13039/100013316, name: Strategic Environmental Research and Development Program
- Language
- English
- Date published
- 11/20/2020
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Center for Biocatalysis and Bioprocessing
- Record Identifier
- 9983997987002771
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