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Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
Journal article   Open access   Peer reviewed

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Julian Wolf, Teja Chemudupati, Aarushi Kumar, Ditte K. Rasmussen, Karen M. Wai, Robert T. Chang, Artis A. Montague, Peter H. Tang, Alexander G. Bassuk, Antoine Dufour, …
Journal of visualized experiments, Vol.199, e65804
09/11/2023
DOI: 10.3791/65804
url
https://doi.org/10.3791/65804View
Published (Version of record) Open Access

Abstract

A critical challenge in translational research is establishing a viable and efficient interface between patient care in the operating room (OR) and the research laboratory. Here, we developed a protocol for acquiring high-quality liquid biopsies for molecular analyses from the aqueous humor and the vitreous from patients undergoing eye surgery. In this workflow, a Mobile Operating Room Lab Interface (MORLI) cart equipped with a computer, a barcode scanner, and lab instruments, including onboard cold storage, is used to obtain and archive human biological samples. A web-based data privacy-compliant database enables annotating each sample over its lifetime, and a cartesian coordinate system allows tracking each barcoded specimen in storage, enabling quick and accurate retrieval of samples for downstream analyses. Molecular characterization of human tissue samples not only serves as a diagnostic tool (e.g., to distinguish between infectious endophthalmitis and other non-infectious intraocular inflammation) but also represents an important component of translational research, allowing the identification of new drug targets, development of new diagnostic tools, and personalized therapeutics.

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