Journal article
Biochanin A reduces pancreatic cancer survival and progression
Anti-cancer drugs, Vol.25(3), pp.296-302
03/2014
DOI: 10.1097/CAD.0000000000000044
PMID: 24201306
Abstract
Pancreatic cancer has dismally low mean survival rates worldwide. Only a few chemotherapeutic agents including gemcitabine have been shown to improve the survival of pancreatic cancer patients. Biochanin A, an isoflavone, is known to exert an anticancer effect on various cancer types. In this study, we examined the anticancer properties of biochanin A on pancreatic cancer cells. The effect of biochanin A on cellular survival, apoptosis, and proliferation was analyzed using MTT, flow cytometry, and colony formation assay. The effect of biochanin A on pancreatic cancer's mitogenic signaling was determined using western blot analysis. Migration assay and zymography were used to determine biochanin A's effect on pancreatic cancer progression. Biochanin A induced dose-dependent toxicity on pancreatic cancer cells (Panc1 and AsPC-1). It reduced colony formation ability of Panc1 cells and induced dose-dependent apoptosis. Activation of Akt and MAPK was inhibited. Furthermore, the migratory and invasive potential of the cancer cells was also reduced. The results suggest that biochanin A is effective in reducing pancreatic cancer cell survival by inhibiting their proliferation and inducing apoptosis. It affects mitogenic, migratory, and invasive processes involved in cancer progression. These findings may lead to novel approaches to treat pancreatic cancer using isoflavones in combination with other therapeutic drugs.
Details
- Title: Subtitle
- Biochanin A reduces pancreatic cancer survival and progression
- Creators
- Vikas BhardwajSatya Murthy TadinadaAditi Jain - University of KentuckyVikas SehdevChristopher K DanielsJames C K LaiAlok Bhushan
- Resource Type
- Journal article
- Publication Details
- Anti-cancer drugs, Vol.25(3), pp.296-302
- DOI
- 10.1097/CAD.0000000000000044
- PMID
- 24201306
- NLM abbreviation
- Anticancer Drugs
- ISSN
- 0959-4973
- eISSN
- 1473-5741
- Grant note
- P20 RR016454 / NCRR NIH HHS
- Language
- English
- Date published
- 03/2014
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984966693902771
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