Journal article
Biochemical and Biological Properties of Staphylococcal Enterotoxin K
Infection and immunity, Vol.69(1), pp.360-366
01/2001
DOI: 10.1128/IAI.69.1.360-366.2001
PMCID: PMC97891
PMID: 11119525
Abstract
Staphylococcus aureus
is an important human pathogen which is implicated in a wide variety of diseases. Major determinants of the virulence of this organism include extracellular virulence factors. Staphylococcal enterotoxins (SEs) are important causative agents in staphylococcal toxic shock syndrome and food poisoning. Our study identified a novel enterotoxin, SEK, and examined its biochemical and biological properties. SEK had a molecular weight of 26,000 and an experimentally determined pI of between 7.0 and 7.5. SEK was secreted by clinical isolates of
S. aureus
. We demonstrated that SEK had many of the biological activities associated with the SEs, including superantigenicity, pyrogenicity, the ability to enhance the lethal effect of endotoxin, and lethality in a rabbit model when administered by subcutaneous miniosmotic pump. Recombinant SEK was shown to stimulate human CD4
+
and CD8
+
T cells in a Vβ-specific manner; T-cells bearing Vβ 5.1, 5.2, and 6.7 were significantly stimulated to proliferate.
Details
- Title: Subtitle
- Biochemical and Biological Properties of Staphylococcal Enterotoxin K
- Creators
- Paul M Orwin - ; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016Donald Y. M Leung - ; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016Heather L Donahue - ; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016Richard P Novick - ; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016Patrick M Schlievert - ; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.69(1), pp.360-366
- DOI
- 10.1128/IAI.69.1.360-366.2001
- PMID
- 11119525
- PMCID
- PMC97891
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 01/2001
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001206802771
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