Biocompatibility of Multi-Imaging Engineered Mesoporous Silica Nanoparticles: In Vitro and Adult and Fetal In Vivo Studies

Sean Sweeney; Andrea Adamcakova-Dodd; Peter S Thorne; Jose G Assouline

doi:10.1166/jbn.2017.2369

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Biocompatibility of Multi-Imaging Engineered Mesoporous Silica Nanoparticles: In Vitro and Adult and Fetal In Vivo Studies

Journal article

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Biocompatibility of Multi-Imaging Engineered Mesoporous Silica Nanoparticles: In Vitro and Adult and Fetal In Vivo Studies

Sean Sweeney, Andrea Adamcakova-Dodd, Peter S Thorne and Jose G Assouline

Journal of biomedical nanotechnology, Vol.13(5), pp.544-558

05/2017

DOI: 10.1166/jbn.2017.2369

PMCID: PMC6527114

PMID: 31118876

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https://www.ncbi.nlm.nih.gov/pmc/articles/6527114View

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Abstract

Despite potentially serious adverse effects of engineered nanoparticles on maternal health and fetal development, little is known about their transport across the placenta. Human and animal studies are primarily limited to ex vivo approaches; the lack of a real-time, minimally invasive tool to study transplacental transport is clear. We have developed functionalized mesoporous silica nanoparticles (MSN) for use in magnetic resonance, ultrasound, and fluorescent imaging. This material is designed as a model for, or a carrier of, environmental toxicants, allowing for in vivo evaluation. To establish a baseline of biocompatibility, we present data describing MSN tolerance using in vitro and in vivo models. In cultured cells, MSN were tolerated to a dose of 125 µ g/mL with minimal effect on viability and doubling time. For the 42 day duration of the study, none of the mice exhibited behaviors usually indicative of distress (lethargy, anemia, loss of appetite, etc.). In gravid mice, the body and organ weights of MSN-exposed dams were equivalent to those of control dams. Embryos exposed to MSN during early gestation were underweight by a small degree, while embryos exposed during late gestation were of a slightly larger weight. The rate of spontaneous fetal resorptions were equivalent in exposed and control mice. Maternal livers and sera were screened for a complement of cytokines/chemokines and reactive oxygen/nitrogen species (ROS/RNS). Only granulocyte-colony stimulating factor was elevated in mice exposed to MSN during late gestation, while ROS/RNS levels were elevated in mice exposed during early/mid gestation. These findings may usher future experiments investigating environmental toxicants using real-time assessment of transport across the placenta.

Magnetic Resonance Imaging

Biocompatibility

Placenta

Maternal-Fetal Medicine

Reactive Oxygen Species

Mesoporous Silica Nanoparticles

Details

Title: Subtitle: Biocompatibility of Multi-Imaging Engineered Mesoporous Silica Nanoparticles: In Vitro and Adult and Fetal In Vivo Studies
Creators: Sean Sweeney - NanoMedTrix, Department of Biomedical Engineering, University of Iowa, 227 Engineering Research Facility, Iowa City, IA 52242
Andrea Adamcakova-Dodd - NanoMedTrix, Department of Biomedical Engineering, University of Iowa, 227 Engineering Research Facility, Iowa City, IA 52242
Peter S Thorne - NanoMedTrix, Department of Biomedical Engineering, University of Iowa, 227 Engineering Research Facility, Iowa City, IA 52242
Jose G Assouline - NanoMedTrix, Department of Biomedical Engineering, University of Iowa, 227 Engineering Research Facility, Iowa City, IA 52242
Resource Type: Journal article
Publication Details: Journal of biomedical nanotechnology, Vol.13(5), pp.544-558
DOI: 10.1166/jbn.2017.2369
PMID: 31118876
PMCID: PMC6527114
NLM abbreviation: J Biomed Nanotechnol
ISSN: 1550-7033
eISSN: 1550-7041
Language: English
Date published: 05/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Urology
Record Identifier: 9983997335902771

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