Journal article
Biodegradable particles as vaccine delivery systems: size matters
The AAPS journal, Vol.15(1), pp.85-94
01/2013
DOI: 10.1208/s12248-012-9418-6
PMCID: PMC3535111
PMID: 23054976
Abstract
Poly(lactide-co-glycolide) (PLGA) particles have strong potential as antigen delivery systems. The size of PLGA particles used to vaccinate mice can affect the magnitude of the antigen-specific immune response stimulated. In this study, we fabricated and characterized 17 μm, 7 μm, 1 μm, and 300 nm PLGA particles coloaded with a model antigen ovalbumin (OVA) and CpG oligodeoxynucleotides (CpG ODN). PLGA particles demonstrated a size-dependent burst release followed by a more sustained release of encapsulated molecules. PLGA particles that were 300 nm in size showed the highest internalization by, and maximum activation of, dendritic cells. The systemic antigen-specific immune response to vaccination was measured after administration of two intraperitoneal injections, 7 days apart, of 100 μg OVA and 50 μg CpG ODN in C57BL/6 mice. In vivo studies showed that 300 nm sized PLGA particles generated the highest antigen-specific cytotoxic T cell responses by days 14 and 21. These mice also showed the highest IgG2a:IgG1 ratio of OVA-specific antibodies on day 28. This study suggests that the smaller the PLGA particle used to deliver antigen and adjuvants the stronger the antigen-specific cytotoxic T cell response generated.
Details
- Title: Subtitle
- Biodegradable particles as vaccine delivery systems: size matters
- Creators
- Vijaya B Joshi - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USASean M GearyAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- The AAPS journal, Vol.15(1), pp.85-94
- DOI
- 10.1208/s12248-012-9418-6
- PMID
- 23054976
- PMCID
- PMC3535111
- NLM abbreviation
- AAPS J
- ISSN
- 1550-7416
- eISSN
- 1550-7416
- Publisher
- United States
- Grant note
- P30 ES005605 / NIEHS NIH HHS R21 CA128414 / NCI NIH HHS 1R21CA128414-01A2/UI / NCI NIH HHS
- Language
- English
- Date published
- 01/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983986403902771
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