Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium

Nicholas V Coleman; Timothy E Mattes; James M Gossett; Jim C Spain

doi:10.1128/AEM.68.6.2726-2730.2002

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Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium

Journal article

Open access

Peer reviewed

Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium

Nicholas V Coleman, Timothy E Mattes, James M Gossett and Jim C Spain

Applied and environmental microbiology, Vol.68(6), pp.2726-2730

06/2002

DOI: 10.1128/AEM.68.6.2726-2730.2002

PMCID: PMC123969

PMID: 12039726

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Abstract

An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a beta-proteobacterium. At 20 degrees C, strain JS666 grew on cDCE with a minimum doubling time of 73 +/- 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 +/- 0.2 microM, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.

Thermodynamics

Biodegradation, Environmental

Proteobacteria - classification

Proteobacteria - metabolism

Proteobacteria - growth & development

Dichloroethylenes - metabolism

Details

Title: Subtitle: Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium
Creators: Nicholas V Coleman - Air Force Research Laboratory-MLQL, Tyndall AFB, Florida 32403, USA
Timothy E Mattes
James M Gossett - Cornell University
Jim C Spain
Resource Type: Journal article
Publication Details: Applied and environmental microbiology, Vol.68(6), pp.2726-2730
DOI: 10.1128/AEM.68.6.2726-2730.2002
PMID: 12039726
PMCID: PMC123969
NLM abbreviation: Appl Environ Microbiol
ISSN: 0099-2240
eISSN: 1098-5336
Publisher: United States
Language: English
Date published: 06/2002
Academic Unit: Civil and Environmental Engineering
Record Identifier: 9983991966802771

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