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Biodegradation of mono-hydroxylated PCBs by Burkholderia xenovorans
Journal article   Peer reviewed

Biodegradation of mono-hydroxylated PCBs by Burkholderia xenovorans

Rouzbeh Tehrani, Monica M Lyv, Rashid Kaveh, Jerald L Schnoor and Benoit Van Aken
Biotechnology letters, Vol.34(12), pp.2247-2252
12/2012
DOI: 10.1007/s10529-012-1037-x
PMCID: PMC3592572
PMID: 22918793
url
https://www.ncbi.nlm.nih.gov/pmc/articles/3592572View
Open Access

Abstract

Three hydroxylated derivatives of PCBs, 2'-hydroxy-4-chlorobiphenyl (2'-OH-4-CB), 3'-hydroxy-4-chlorobiphenyl (3'-OH-4-CB), and 4'-hydroxy-4-chlorobiphenyl (4'-OH-4-CB), were transformed by the PCB degrader, Burkholderia xenovorans. When the bacterium was growing on biphenyl (biphenyl pathway-inducing conditions), all three hydroxylated isomers were transformed. However, only 2'-OH-4-CB was transformed by the bacterium growing on succinate (conditions non-inductive of the biphenyl pathway). Gene expression analyses showed a strong induction of key genes of the biphenyl pathway (bph) when cells were grown on biphenyl, which is consistent with the transformation of the three isomers by biphenyl-grown cells. When cells were grown on succinate, only exposure to 2'-OH-4-CB resulted in expression of biphenyl pathway genes, which suggests that this isomer was capable of inducing the biphenyl pathway. These results provide the first evidence that bacteria are able to metabolize PCB derivatives hydroxylated on the non-chlorinated ring.
 Burkholderia - genetics Metabolic Networks and Pathways - genetics Burkholderia - metabolism Hydroxylation Succinic Acid - metabolism Biotransformation Gene Expression Profiling Polychlorinated Biphenyls - metabolism Biphenyl Compounds - metabolism Burkholderia - growth & development

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