Journal article
Biodistribution of indium-111-labeled antibody directed against intercellular adhesion molecule-1
The Journal of nuclear medicine (1978), Vol.37(4), pp.656-661
1996
PMID: 8691262
Abstract
We examined the biodistribution in normal rats of an 111In-labeled mouse monoclonal antibody to rat intercellular adhesion molecule-1 (111In-alCAM-1), as a potential detector of inflammation.
Methods: Indium-111-alCAM-1 or 111In-labeled normal mouse polyclonal immunoglobulin G (111In-nmIgG) was injected into rats. Groups of three to four rats were killed up to 18 hr after injection, and activity was measured in various tissues. Rats were also imaged at 1 and 18 hr after injection.
Results: Uptake of 111In-alCAM-1 was greatest in the lung (approximately 10% injected dose [ID]/g at 15 min) and then declined steadily (to approximately 2% ID/g at 18 hr). Lung uptake of 111In-nmIgG was eightfold less than that for 111In-alCAM-1 and did not change throughout the 18 hr. At all time points, blood activity for 111In-alCAM-1 was only 30% to 40% of that for 111In-nmIgG, whereas the percent injected dose per gram was increased more than twofold in the major organs. Compared with 111In-nmIgG, the 111In of alCAM-1 was shifted from the blood and was distributed among the lung kidney, spleen and liver.
Conclusion: Indium-111-alCAM-1 may be useful as an early inflammation detection agent. Intercellular adhesion molecule-1 upregulation is a very early event in inflammation and rapid removal from the blood of this antibody provides low background in contrast to the usual high background with whole antibodies.
Details
- Title: Subtitle
- Biodistribution of indium-111-labeled antibody directed against intercellular adhesion molecule-1
- Creators
- Daniel E Sasso - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United StatesMaria A Gionfriddo - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United StatesRoger S Thrall - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United StatesSergei I Syrbu - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United StatesHenry M Smilowitz - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United StatesRonald E Weiner - Departments of Nuclear Medicine, Medicine, Surgery and Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of nuclear medicine (1978), Vol.37(4), pp.656-661
- Publisher
- Society of Nuclear Medicine; Reston, VA
- PMID
- 8691262
- ISSN
- 0161-5505
- eISSN
- 1535-5667
- Language
- English
- Date published
- 1996
- Academic Unit
- Pathology
- Record Identifier
- 9984047876702771
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