Journal article
Bioenergetics of murine lungs infected with respiratory syncytial virus
Virology journal, Vol.10(1), pp.22-22
2013
DOI: 10.1186/1743-422X-10-22
PMCID: PMC3616819
PMID: 23320837
Abstract
Background: Cellular bioenergetics (cellular respiration and accompanying ATP synthesis) is a highly sensitive biomarker of tissue injury and may be altered following infection. The status of cellular mitochondrial O(2) consumption of the lung in pulmonary RSV infection is unknown.
Methods: In this study, lung fragments from RSV-infected BALB/c mice were evaluated for cellular O(2) consumption, ATP content and caspase activity. The disease was induced by intranasal inoculation with the RSV strain A2 and lung specimens were analyzed on days 2-15 after inoculation. A phosphorescence O(2) analyzer that measured dissolved O(2) concentration as a function of time was used to monitor respiration. The caspase-3 substrate analogue N-acetyl-asp-glu-val-asp-7-amino-4-methylcoumarin (Ac-DEVD-AMC) was used to monitor intracellular caspases.
Results: O(2) concentration declined linearly with time when measured in a sealed vial containing lung fragment and glucose as a respiratory substrate, revealing its zero-order kinetics. O(2) consumption was inhibited by cyanide, confirming the oxidation occurred in the respiratory chain. Cellular respiration increased by 1.6-fold (p<0.010) and ATP content increased by 3-fold in the first week of RSV infection. Both parameters returned to levels found in uninfected lungs in the second week of RSV infection. Intracellular caspase activity in infected lungs was similar to uninfected lungs throughout the course of disease.
Conclusions: Lung tissue bioenergetics is transiently enhanced in RSV infection. This energy burst, triggered by the virus or virus-induced inflammation, is an early biomarker of the disease and may be targeted for therapy.
Details
- Title: Subtitle
- Bioenergetics of murine lungs infected with respiratory syncytial virus
- Creators
- Ahmed R Alsuwaidi - Departments of Pediatrics, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesSheela Benedict - Departments of Pediatrics, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesJose Kochiyil - Departments of Pediatrics, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesFarah Mustafa - Departments of Biochemistry, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesStacey M Hartwig - Department of Microbiology, University of Iowa, Iowa City, IA, 52242, USASaeeda Almarzooqi - Departments of Pathology, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesAlia Albawardi - Departments of Pathology, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesTahir A Rizvi - Departments of Microbiology, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab EmiratesSteven M Varga - Department of Microbiology, University of Iowa, Iowa City, IA, 52242, USAAbdul-Kader Souid - Departments of Pediatrics, College of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, United Arab Emirates
- Resource Type
- Journal article
- Publication Details
- Virology journal, Vol.10(1), pp.22-22
- Publisher
- BioMed Central
- DOI
- 10.1186/1743-422X-10-22
- PMID
- 23320837
- PMCID
- PMC3616819
- ISSN
- 1743-422X
- eISSN
- 1743-422X
- Language
- English
- Date published
- 2013
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083884602771
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