Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material

Katherine J Lauderdale; Cheryl L Malone; Blaise R Boles; Jose Morcuende; Alexander R Horswill

doi:10.1002/jor.20943

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Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material

Journal article

Open access

Peer reviewed

Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material

Katherine J Lauderdale, Cheryl L Malone, Blaise R Boles, Jose Morcuende and Alexander R Horswill

Journal of orthopaedic research, Vol.28(1), pp.55-61

01/2010

DOI: 10.1002/jor.20943

PMID: 19610092

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Abstract

Orthopedic implant-related bacterial infections are associated with high morbidity that may lead to limb amputation and exert significant financial burden on the healthcare system. Staphylococcus aureus is a dominant cause of these infections, and increased incidence of community-associated methicillin-resistant S. aureus (CA-MRSA) is being reported. The ability of S. aureus to attach to the foreign body surface and develop a biofilm is an important determinant of resistance to antibiotic prophylaxis. To gain insight on CA-MRSA biofilm properties, USA300 biofilm maturation and dispersal was examined, and these biofilms were found to exhibit pronounced, quorum-sensing mediated dispersal from a glass surface. For comparison of biofilm maturation on different surface chemistries, USA300 biofilm growth was examined on glass, polycarbonate, and titanium, and minimal differences were apparent in thickness, total biomass, and substratum coverage. Importantly, USA300 biofilms grown on titanium possessed a functional dispersal mechanism, and the dispersed cells regained susceptibility to rifampicin and levofloxacin treatment. The titanium biofilms were also sensitive to proteinase K and DNaseI, suggesting the matrix is composed of proteinaceous material and extracellular DNA. These studies provide new insights on the properties of CA-MRSA biofilms on implant materials, and indicate that quorum-sensing dispersion could be an effective therapeutic strategy.

Methicillin-Resistant Staphylococcus aureus - physiology

Quorum Sensing

Humans

Biofilms - growth & development

Prosthesis Design

Internal Fixators - microbiology

Prosthesis-Related Infections - prevention & control

Arthroplasty, Replacement - instrumentation

Prosthesis-Related Infections - microbiology

Models, Biological

Surface Properties

Titanium

Biocompatible Materials

Staphylococcal Infections - prevention & control

Community-Acquired Infections - prevention & control

Staphylococcal Infections - microbiology

Community-Acquired Infections - microbiology

Details

Title: Subtitle: Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material
Creators: Katherine J Lauderdale - Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Cheryl L Malone
Blaise R Boles
Jose Morcuende
Alexander R Horswill
Resource Type: Journal article
Publication Details: Journal of orthopaedic research, Vol.28(1), pp.55-61
DOI: 10.1002/jor.20943
PMID: 19610092
NLM abbreviation: J Orthop Res
ISSN: 0736-0266
eISSN: 1554-527X
Publisher: United States
Grant note: R01 AI078921 / NIAID NIH HHS AI078921 / NIAID NIH HHS
Language: English
Date published: 01/2010
Academic Unit: Stead Family Department of Pediatrics; Orthopedics and Rehabilitation
Record Identifier: 9984040327502771

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