Biofilm microbial communities of denture stomatitis

M S Campos; L Marchini; L A S Bernardes; L C Paulino; F G Nobrega

doi:10.1111/j.1399-302X.2008.00445.x

Back

Journal article

Biofilm microbial communities of denture stomatitis

M S Campos, L Marchini, L A S Bernardes, L C Paulino and F G Nobrega

Oral microbiology and immunology, Vol.23(5), pp.419-424

10/2008

DOI: 10.1111/j.1399-302X.2008.00445.x

PMID: 18793366

View Online

Abstract

Denture stomatitis is a common lesion that affects denture wearers. Its multifactorial etiology seems to depend on a complex and poorly characterized biofilm. The purpose of this study was to assess the composition of the microbial biofilm obtained from complete denture wearers with and without denture stomatitis using culture-independent methods. Samples were collected from healthy denture wearers and from patients with denture stomatitis. Libraries comprising about 600 cloned 16S ribosomal DNA (rDNA) bacterial sequences and 192 cloned eukaryotic internal transcribed spacer (ITS) region sequences, obtained by polymerase chain reactions, were analyzed. The partial 16S rDNA sequences revealed a total of 82 bacterial species identified in healthy subjects and patients with denture stomatitis. Twenty-seven bacterial species were detected in both biofilms, 29 species were exclusively present in patients with denture stomatitis, and 26 were found only in healthy subjects. Analysis of the ITS region revealed the presence of Candida sp. in both biofilms. The results revealed the extent of the microbial flora, suggesting the existence of distinct biofilms in healthy subjects and in patients with denture stomatitis.

DNA, Fungal - analysis

DNA, Ribosomal - analysis

Humans

Middle Aged

RNA, Ribosomal, 16S - analysis

Male

Actinobacteria - classification

Candida tropicalis - classification

Candida - classification

DNA, Ribosomal Spacer - analysis

Streptococcus - classification

Veillonella - classification

Candida glabrata - classification

Stomatitis, Denture - microbiology

Polymerase Chain Reaction

DNA, Bacterial - analysis

Female

Denture, Complete - microbiology

Biofilms - classification

Prevotella - classification

Details

Title: Subtitle: Biofilm microbial communities of denture stomatitis
Creators: M S Campos - Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil. mscamp@usp.br
L Marchini
L A S Bernardes
L C Paulino
F G Nobrega
Resource Type: Journal article
Publication Details: Oral microbiology and immunology, Vol.23(5), pp.419-424
Publisher: Denmark
DOI: 10.1111/j.1399-302X.2008.00445.x
PMID: 18793366
ISSN: 0902-0055
eISSN: 1399-302X
Language: English
Date published: 10/2008
Academic Unit: Preventive and Community Dentistry; Oral Pathology, Radiology and Medicine
Record Identifier: 9983806381502771

Metrics

31 Record Views

79 Times Cited - Web of Science

See more details