Journal article
Biological Correlations and Confounders for Quantification of Retinal Ganglion Cells by Optical Coherence Tomography Based on Studies of Outbred Mice
Translational vision science & technology, Vol.11(9), pp.17-17
09/01/2022
DOI: 10.1167/tvst.11.9.17
PMCID: PMC9513741
PMID: 36135979
Abstract
PurposeDespite popularity of optical coherence tomography (OCT) in glaucoma studies, it's unclear how well OCT-derived metrics compare to traditional measures of retinal ganglion cell (RGC) abundance. Here, Diversity Outbred (J:DO) mice are used to directly compare ganglion cell complex (GCC) thickness measured by OCT to metrics of retinal anatomy measured ex vivo with retinal wholemounts and optic nerve histology. MethodsJ:DO mice (n = 48) underwent fundoscopic and OCT examinations, with automated segmentation of GCC thickness. RGC axons were quantified from para-phenylenediamine-stained optic nerve cross-sections and somas from BRN3A-immunolabeled retinal wholemounts, with total inner retinal cellularity assessed by TO-PRO and subsequent hematoxylin staining. ResultsJ:DO tissues lacked overt disease. GCC thickness, RGC abundance, and total cell abundance varied broadly across individuals. GCC thickness correlated significantly to RGC somal density (r = 0.58) and axon number (r = 0.44), but not total cell density. Retinal area and nerve cross-sectional area varied widely. No metrics were significantly influenced by sex. In bilateral comparisons, GCC thickness (r = 0.95), axon (r = 0.72), and total cell density (r = 0.47) correlated significantly within individuals. ConclusionsAmongst outbred mice, OCT-derived measurements of GCC thickness correlate significantly to RGC somal and axon abundance. Factors limiting correlation are likely both biological and methodological, including differences in retinal area that distort sampling-based estimates of RGC abundance. Translational RelevanceThere are significant-but imperfect-correlations between GCC thickness and RGC abundance across genetic contexts in mice, highlighting valid uses and ongoing challenges for meaningful use of OCT-derived metrics.
Details
- Title: Subtitle
- Biological Correlations and Confounders for Quantification of Retinal Ganglion Cells by Optical Coherence Tomography Based on Studies of Outbred Mice
- Creators
- Adam Hedberg-Buenz - University of IowaKacie J Meyer - University of IowaCarly J van der Heide - University of IowaWenxiang Deng - University of IowaKyungmoo Lee - University of IowaDana A Soukup - University of IowaMonica Kettelson - University of IowaDanielle Pellack - University of IowaHannah Mercer - University of IowaKai Wang - University of IowaMona K Garvin - University of IowaMichael D Abramoff - University of IowaMichael G Anderson - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Translational vision science & technology, Vol.11(9), pp.17-17
- DOI
- 10.1167/tvst.11.9.17
- PMID
- 36135979
- PMCID
- PMC9513741
- NLM abbreviation
- Transl Vis Sci Technol
- eISSN
- 2164-2591
- Language
- English
- Date published
- 09/01/2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Molecular Physiology and Biophysics; Psychiatry; Biostatistics; Fraternal Order of Eagles Diabetes Research Center; Ophthalmology and Visual Sciences
- Record Identifier
- 9984297159202771
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