Journal article
Biological Differences in rAAV Transduction of Airway Epithelia in Humans and in Old World Non-human Primates
Molecular therapy, Vol.15(12), pp.2114-2123
12/2007
DOI: 10.1038/sj.mt.6300277
PMCID: PMC2121582
PMID: 17667945
Abstract
Non-human primates (NHPs) are considered to be among the most relevant animal models for pre-clinical testing of human therapies, on the basis of their close evolutionary relatedness to humans in terms of organ cell biology and physiology. In this study, we sought to investigate whether NHP models accurately reflect the effectiveness of recombinant adeno-associated virus (rAAV)-mediated gene delivery to the airway in humans. In order to do this, we utilized an identical model system of differentiated airway epithelia from Indian Rhesus monkeys and from humans, cultured at an air–liquid interface (ALI). In addition to assessing the biology of rAAV-mediated transduction for three serotypes, we characterized the bioelectric properties as a reference for biological similarities and differences between the cell cultures from the two species. Our results demonstrate that airway epithelia from NHPs and humans have very similar Na+ and Cl− transport properties. In contrast, rAAV transduction of airway epithelia of NHPs demonstrated significant differences to those in humans with regard to the efficiency of apical and/or basal transduction with three rAAV serotypes (AAV1, AAV2, AAV5). These findings suggest that the IndianRhesusmonkey may not be the best model for preclinical testing of rAAV-mediated gene therapy to the airway in humans.
Details
- Title: Subtitle
- Biological Differences in rAAV Transduction of Airway Epithelia in Humans and in Old World Non-human Primates
- Creators
- Xiaoming Liu - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa, USAMeihui Luo - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa, USACyndi Trygg - Center for Gene Therapy and Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana, USAZiying Yan - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa, USADiana CM Lei-Butters - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa, USACarolina I Smith - Department of Physiology, Johns Hopkins University, Baltimore, Maryland, USAAnne C Fischer - Department of Surgery, Johns Hopkins University, Baltimore, Maryland, USAKeith Munson - Targeted Genetics Corporation, Seattle, Washington, USAWilliam B Guggino - Department of Physiology, Johns Hopkins University, Baltimore, Maryland, USABruce A Bunnell - Center for Gene Therapy and Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana, USAJohn F Engelhardt - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Molecular therapy, Vol.15(12), pp.2114-2123
- Publisher
- Elsevier Inc
- DOI
- 10.1038/sj.mt.6300277
- PMID
- 17667945
- PMCID
- PMC2121582
- ISSN
- 1525-0016
- eISSN
- 1525-0024
- Language
- English
- Date published
- 12/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025420502771
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