Journal article
Biological Effects of Pseudomonas aeruginosa Type III-Secreted Proteins on CHO Cells
Infection and immunity, Vol.67(4), pp.2040-2044
Note
04/1999
DOI: 10.1128/.67.4.2040-2044.1999
PMCID: PMC96567
PMID: 10085057
Abstract
A strain of
Pseudomonas aeruginosa
that fails to express known type III-secreted effector proteins was constructed as an expression host. Individual effectors were expressed in
trans
, and their biological effects on CHO cells were assessed in an acute cellular infection model. Intoxication with ExoS, ExoT, or ExoY resulted in alterations in cell morphology. As shown in previous genetic studies, ExoU expression was linked to acute cytotoxicity.
Details
- Title: Subtitle
- Biological Effects of Pseudomonas aeruginosa Type III-Secreted Proteins on CHO Cells
- Creators
- Amy J Vallis - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226Viviane Finck-Barbançon - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226Timothy L Yahr - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226Dara W Frank - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.67(4), pp.2040-2044
- Series
- Note
- DOI
- 10.1128/.67.4.2040-2044.1999
- PMID
- 10085057
- PMCID
- PMC96567
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 04/1999
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002383002771
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