Journal article
Biological Effects of Recombinant Human Granulocyte Colony-Stimulating Factor in Patients With Untreated Acute Myeloid Leukemia
Blood, Vol.87(4), pp.1484-1494
02/15/1996
DOI: 10.1182/blood.V87.4.1484.bloodjournal8741484
PMID: 8608239
Abstract
Hematopoietic growth factors are being administered to patients with acute myeloid leukemia (AMD both to shorten the duration of chemotherapy-induced neutropenia and in an attempt to increase cytotoxicity of cell cycle-specific agents. However, limited information is available concerning the effects of growth factors in AML patients. To examine the in vivo effects of recombinant human granulocyte colony-stimulating factor (G-CSF) on AML cells, laboratory studies were performed before and after a 72-hour intravenous infusion of G-CSF (10 μg/kg/d) administered to 28 untreated AML patients. Twenty-seven patients (96%) showed increases in at least one of the following parameters after G-CSF: blood blasts, bone marrow (BM) blasts, leukemia cells in S phase or interphase cells with leukemia-specific markers shown by fluorescence in situ hybridization. The median paired change in absolute blast count was +2.7 × 109/L (P = .0001) after G-CSF, as compared with 0.0 during the 72 hours before initiation of G-CSF. The median percentage of BM leukemia cells in S phase increased from 6.0% to 10.7% after G-CSF (median change, +5.9%; P = .009). Interphase BM cells with trisomy 8 or monosomy 7 increased in 6 of 6 patients with these abnormalities (P = .02), with a median percent increase of 47%. Blood neutrophil counts also increased during G-CSF (median paired change, +2.8 × 109/L; P < .0001). Trisomy 8 or monosomy 7 was shown by fluorescence in situ hybridization in post–G-CSF blood neutrophils from 4 of 6 patients but was also present in neutrophils before G-CSF. We conclude that the percentage of leukemia cells in S phase increases and that leukemic cell populations undergo expansion during short-term administration of G-CSF in almost all AML patients.
Details
- Title: Subtitle
- Biological Effects of Recombinant Human Granulocyte Colony-Stimulating Factor in Patients With Untreated Acute Myeloid Leukemia
- Creators
- Maria R. Baer - Roswell Park Cancer InstituteSteven H. Bernstein - Baylor University Medical CenterVirginia L. Brunetto - Baylor University Medical CenterKristiina Heinonen - Baylor University Medical CenterKrzysztof Mrózek - Baylor University Medical CenterVictoria L. Swann - Baylor University Medical CenterHans Minderman - Roswell Park Cancer InstituteAnneMarie W. Block - Baylor University Medical CenterLynda A. Pixley - Baylor University Medical CenterNeal P. Christiansen - Baylor University Medical CenterJoseph W. Fay - Baylor University Medical CenterMaurice Barcos - Baylor University Medical CenterYoucef Rustum - Baylor University Medical CenterGeoffrey P. Herzig - Baylor University Medical CenterClara D. Bloomfield - Baylor University Medical Center
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.87(4), pp.1484-1494
- Publisher
- Elsevier Inc
- DOI
- 10.1182/blood.V87.4.1484.bloodjournal8741484
- PMID
- 8608239
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 02/15/1996
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359895702771
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