Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1

Gregory A Bohach; Jeffrey P Handley; Patrick M Schlievert

doi:10.1128/IAI.57.1.23-28.1989

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Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1

Journal article

Open access

Peer reviewed

Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1

Gregory A Bohach, Jeffrey P Handley and Patrick M Schlievert

Infection and immunity, Vol.57(1), pp.23-28

01/1989

DOI: 10.1128/IAI.57.1.23-28.1989

PMCID: PMC313035

PMID: 2909489

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Abstract

Comparisons of recently published primary sequences of staphylococcal and streptococcal pyrogenic toxins prompted an evaluation of biological and immunological properties of the C terminus of staphylococcal enterotoxin C1. The 59 N-terminal amino acids were deleted from the toxin by digestion with trypsin. The resulting fragment (Mr, 20,659) contained the remaining 180 C-terminal residues. This fragment (Trp F1) consisted of two polypeptide chains (Trp F1a and Trp F1b) linked by cysteine residues. Trp F1 was mitogenic, pyrogenic, and enhanced susceptibility of rabbits to lethal endotoxin shock. In addition, this fragment contained at least one antigenic epitope that cross-reacted with enterotoxin B.

Amino Acid Sequence

Rabbits

Peptide Fragments - isolation & purification

Enterotoxins - physiology

Antigens, Bacterial - immunology

Molecular Sequence Data

Neutralization Tests

Enterotoxins - isolation & purification

Cross Reactions

Peptide Fragments - physiology

Trypsin

Animals

Enterotoxins - immunology

Peptide Mapping

Peptide Fragments - immunology

Antibodies, Bacterial - immunology

Staphylococcus aureus - immunology

Mice

Mice, Inbred BALB C

Mitogens - physiology

Antibodies, Monoclonal - immunology

Details

Title: Subtitle: Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1
Creators: Gregory A Bohach - Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455
Jeffrey P Handley
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.57(1), pp.23-28
Publisher: United States
DOI: 10.1128/IAI.57.1.23-28.1989
PMID: 2909489
PMCID: PMC313035
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: HI36611 / NHLBI NIH HHS
Language: English
Date published: 01/1989
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001122502771

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