Journal article
Biological function of unannotated transcription during the early development of Drosophila melanogaster
Nature genetics, Vol.38(10), pp.1151-1158
10/2006
DOI: 10.1038/ng1875
PMID: 16951679
Abstract
Many animal and plant genomes are transcribed much more extensively than current annotations predict. However, the biological function of these unannotated transcribed regions is largely unknown. Approximately 7% and 23% of the detected transcribed nucleotides during D. melanogaster embryogenesis map to unannotated intergenic and intronic regions, respectively. Based on computational analysis of coordinated transcription, we conservatively estimate that 29% of all unannotated transcribed sequences function as missed or alternative exons of well-characterized protein-coding genes. We estimate that 15.6% of intergenic transcribed regions function as missed or alternative transcription start sites (TSS) used by 11.4% of the expressed protein-coding genes. Identification of P element mutations within or near newly identified 5' exons provides a strategy for mapping previously uncharacterized mutations to their respective genes. Collectively, these data indicate that at least 85% of the fly genome is transcribed and processed into mature transcripts representing at least 30% of the fly genome.
Details
- Title: Subtitle
- Biological function of unannotated transcription during the early development of Drosophila melanogaster
- Creators
- J Robert Manak - Affymetrix, Inc., Santa Clara, California, 95051, USA. John_Manak@affymetrix.comSujit DikeVictor SementchenkoPhilipp KapranovFrederic BiemarJeff LongJill ChengIan BellSrinka GhoshAntonio PiccolboniThomas R Gingeras
- Resource Type
- Journal article
- Publication Details
- Nature genetics, Vol.38(10), pp.1151-1158
- DOI
- 10.1038/ng1875
- PMID
- 16951679
- NLM abbreviation
- Nat Genet
- ISSN
- 1061-4036
- eISSN
- 1546-1718
- Publisher
- United States
- Grant note
- N01-CO-12400 / NCI NIH HHS U01 HG003147 / NHGRI NIH HHS
- Language
- English
- Date published
- 10/2006
- Academic Unit
- Stead Family Department of Pediatrics; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9983992099202771
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