Journal article
Biome-microbiome interactions in peri-implantitis: A pilot investigation
Journal of periodontology (1970), Vol.93(6), pp.814-823
06/01/2022
DOI: 10.1002/JPER.21-0423
PMCID: PMC9187590
PMID: 35073418
Abstract
Background Dental implants replace missing teeth in at least 100 million people, yet over one million implants fail every year due to peri-implantitis, a bacterially induced inflammatory disease. Our ability to treat peri-implantitis is hampered by a paucity of information on host-microbiome interactions that underlie the disease. Here, we present the first open-ended characterization of transcriptional events at the mucosal-microbial interface in the peri-implant crevice. Methods We simultaneously sequenced microbial and human mRNA from five pairs of healthy and diseased implants from the same patient and used graph theoretics to examine correlations between microbial and host gene expression in the peri-implant crevice. Results We identified a transcriptionally active peri-implant microbiome surrounding healthy implants. Microbial genes encoding phenylalanine, tyrosine, and tryptophan biosynthesis, cysteine, methionine, arginine, proline, and histidine metabolism correlated to human genes encoding cell development, metabolism, morphogenesis, adhesion, gap junctions, cell-cell signaling, and immunoinflammatory pathways, suggesting a role for commensals in protecting epithelial integrity. In disease, we found 4- to 200-fold upregulation in microbial genes encoding biofilm thickness, heme transport and utilization, and Gram-negative cell membrane synthesis. These genes correlated with mucosal zinc finger proteins, apoptosis, membrane transport, inflammation, and cell-cell communication. Conclusions Within the limitations of a small sample size, our data suggest that microbial dysbiosis in the peri-implant sulcus might promote abandonment of host-bacterial transactions that dictate health and instead drive a move towards chronic programming of a non-healing wound.
Details
- Title: Subtitle
- Biome-microbiome interactions in peri-implantitis: A pilot investigation
- Creators
- Sukirth M. Ganesan - The Ohio State UniversityShareef M. Dabdoub - The Ohio State UniversityHaikady N. Nagaraja - The Ohio State UniversityAngelo J. Mariotti - The Ohio State UniversityChristopher W. Ludden - The Ohio State UniversityPurnima S. Kumar - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Journal of periodontology (1970), Vol.93(6), pp.814-823
- DOI
- 10.1002/JPER.21-0423
- PMID
- 35073418
- PMCID
- PMC9187590
- NLM abbreviation
- J Periodontol
- ISSN
- 0022-3492
- eISSN
- 1943-3670
- Publisher
- Wiley
- Number of pages
- 10
- Grant note
- R01 DE022579 / NIDCR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
- Language
- English
- Date published
- 06/01/2022
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984367742002771
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