Journal article
Biomechanical disc culture system: feasibility study using rat intervertebral discs
Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, Vol.225(6), pp.611-620
06/2011
DOI: 10.1177/2041303310394919
PMID: 22034744
Abstract
A small-scale biomechanical disc culture system was designed to stimulate intervertebral disc (IVD) ‘motion segment’ in culture environment with load-controlled compression and combined load (compression + shear). After 7 days of diurnal mechanical loading, cell viability of discs stimulated with static compression load (0.25 MPa) and static combined load (compression (0.25 MPa) + shear (1.5 N)) were similar (> 90 per cent) to unloaded controls. Mechanically stimulated discs showed decrease in static/dynamic moduli, early stress relaxation, and loss of disc height after 7 days of diurnal loading. Histological data of discs indicated load-induced transformations that were not apparent in controls. The feasibility of studying the mechanobiology of intact IVD as a motion segment was demonstrated. Media conditioning (improve tissue stability in long-term culture) and application of biochemical gene expression assays (differential tissue response to types of mechanical stimulation) are proposed as future improvements. The study suggests that the limitations in studying mechanobiology of IVD pathology in vitro can be overcome and it is possible to understand the physiologically relevant mechanism of IVD pathology.
Details
- Title: Subtitle
- Biomechanical disc culture system: feasibility study using rat intervertebral discs
- Creators
- P S Ramakrishnan - Department of Orthopaedic Surgery, University of Iowa, Iowa City, IA, USAJ Hong - Biomechatronics Laboratory, Department of Control and Instrumentation Engineering, Korea University, South KoreaJ A Martin - Department of Orthopaedic Surgery, University of Iowa, Iowa City, IA, USAG L Kurriger - Department of Orthopaedic Surgery, University of Iowa, Iowa City, IA, USAJ A Buckwalter - Department of Orthopaedic Surgery, University of Iowa, Iowa City, IA, USAT-H Lim - Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, Vol.225(6), pp.611-620
- DOI
- 10.1177/2041303310394919
- PMID
- 22034744
- NLM abbreviation
- Proc Inst Mech Eng H
- ISSN
- 0954-4119
- eISSN
- 2041-3033
- Language
- English
- Date published
- 06/2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
- Record Identifier
- 9984040010602771
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