Journal article
Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts
Biomaterials science, Vol.9(22), pp.7565-7574
11/09/2021
DOI: 10.1039/d1bm01119g
PMCID: PMC10547016
PMID: 34664567
Abstract
Polydopamine-assisted modification for bone substitute materials has recently shown great application potential in bone tissue engineering due to its excellent biocompatibility and adhesive properties. A scaffold material's impact on osteoclasts is equally as important as its impact on osteoblasts when considering tissue engineering for bone defect repair, as healthy bone regeneration requires an orchestrated coupling between osteoclasts and osteoblasts. How polydopamine-functionalized bone substitute materials modulate the activity of osteoblast lineage cells has been extensively investigated, but much less is known about their impact on osteoclasts. Moreover, most of the polydopamine-functionalized materials would need to additionally load a biomolecule to exert the modulation on osteoclast activity. Herein, we demonstrated that our biomimetic polydopamine-laced hydroxyapatite collagen (PDHC) scaffold material, which does not need to load additional bioactive agent, is sufficiently able to modulate osteoclast activity in vitro. First, PDHC showed an anti-resorptive potential, characterized by decreased osteoclast differentiation and resorption capacity and changes in osteoclasts' transcriptome profile. Next, cAMP response element-binding protein (CREB) activity was found to mediate PDHC's anti-osteoclastogenic effect. Finally, although PDHC altered clastokines expression pattern of osteoclasts, as revealed by transcriptomic and secretomic analysis, osteoclasts' coupling to osteoblasts was not compromised by PDHC. Collectively, this study demonstrated the PDHC material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts. Such a feature is favorable for the net increase of bone mass, which endows the PDHC material with great application potential in preclinical/clinical bone defect repair.
Details
- Title: Subtitle
- Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts
- Creators
- Lufei Wang - Division of Oral and Craniofacial Health Sciences, University of North Carolina Adams School of Dentistry, Chapel Hill, NC, USA.Tai-Hsien Wu - The Ohio State UniversityXiangxiang Hu - Division of Oral and Craniofacial Health Sciences, University of North Carolina Adams School of Dentistry, Chapel Hill, NC, USA.Jie Liu - The Ohio State UniversityDi Wu - University of North Carolina at Chapel HillPatricia A. Miguez - University of North Carolina at Chapel HillJohn Timothy Wright - University of North Carolina at Chapel HillShaoping Zhang - University of IowaJen-Tsan Chi - Duke UniversityHenry C. Tseng - Duke UniversityChing-Chang Ko - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Biomaterials science, Vol.9(22), pp.7565-7574
- DOI
- 10.1039/d1bm01119g
- PMID
- 34664567
- PMCID
- PMC10547016
- NLM abbreviation
- Biomater Sci
- ISSN
- 2047-4830
- eISSN
- 2047-4849
- Publisher
- Royal Soc Chemistry
- Number of pages
- 10
- Grant note
- R01DE022816 / NIH/NIDCR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) Ohio State University College of Dentistry
- Language
- English
- Date published
- 11/09/2021
- Academic Unit
- Periodontics
- Record Identifier
- 9984367736402771
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