Journal article
Biomonitoring of occupational exposure to styrene in a plastics lamination plant
Mutation research, Vol.428(1), pp.255-269
1999
DOI: 10.1016/S1383-5742(99)00052-6
PMID: 10517998
Abstract
A comprehensive approach to biological monitoring of 44 workers occupationally exposed to styrene in a hand lamination plant was performed by using several end-points: styrene in workplace air, styrene in exhaled air, styrene in blood, DNA strand breaks (SBs) and oxidised bases in mononuclear leukocytes, chromosomal aberrations in lymphocytes, immune parameters and genotyping of polymorphic genes of some xenobiotic-metabolizing enzymes (
CYP 1A1,
EPHX,
GSTM1 and
GSTP1). We found a significantly higher number of DNA SBs, measured by a modified comet assay, in mononuclear leukocytes of the styrene-exposed workers compared with results from 19 unexposed controls (
P<0.001). A fairly strong correlation was observed between SBs and years of exposure (
P<0.001,
r=0.545). The styrene-exposed workers also showed a significantly increased frequency of chromosomal aberrations (
P<0.0001 for highly exposed group,
P<0.004 for medium-exposed group, and
P=0.0001 for low-exposed group). The proliferative response of T-lymphocytes stimulated with concanavalin A was significantly suppressed in people exposed to styrene (
P<0.05). We recorded a significant increase of the percentage of monocytes in differential white blood cell counts in the exposed group (
P<0.05). Using flow cytometry, we found an increased expression of adhesion molecules CD62L, CD18, CD11a, CD11b, CD49d and CD54 in the exposed workers as compared with the control group (
P<0.05).
Details
- Title: Subtitle
- Biomonitoring of occupational exposure to styrene in a plastics lamination plant
- Creators
- M Somorovská - Slovak Medical UniversityE Jahnová - Institute of Preventive and Clinical Medicine SlovakiaJ Tulinská - Institute of Preventive and Clinical Medicine SlovakiaM Zámečnı́ková - National Institute of Public Health, Bratislava, Slovak RepublicJ Šarmanová - National Institute of Public HealthA Terenová - National Institute of Public HealthL Vodičková - National Institute of Public HealthA Lı́šková - Institute of Preventive and Clinical Medicine, Limbová 14, 833 01 Bratislava, Slovak RepublicB Vallová - Institute of Preventive and Clinical Medicine SlovakiaP Souček - National Institute of Public HealthK Hemminki - Karolinska InstitutetH Norppa - Finnish Institute of Occupational HealthA Hirvonen - Finnish Institute of Occupational HealthA.D Tates - Leiden UniversityL Fuortes - University of IowaM Dušinská - Institute of Preventive and Clinical Medicine SlovakiaP Vodička - Czech Academy of Sciences
- Resource Type
- Journal article
- Publication Details
- Mutation research, Vol.428(1), pp.255-269
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S1383-5742(99)00052-6
- PMID
- 10517998
- ISSN
- 0027-5107
- eISSN
- 1873-135X
- Language
- English
- Date published
- 1999
- Academic Unit
- Occupational and Environmental Health; Internal Medicine
- Record Identifier
- 9984364458602771
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