Journal article
Bioreactor Headspace Purging Reduces Dissolved Carbon Dioxide Accumulation in Insect Cell Cultures and Enhances Cell Growth
Biotechnology progress, Vol.13(6), pp.875-877
1997
DOI: 10.1021/bp970078s
Abstract
Flushing the bioreactor headspace of Sf‐9 and High Five insect cell cultures sparged with pure oxygen prevented the accumulation of CO2 in the culture medium and enhanced the cell growth rate and maximum cell densities. The CO2 accumulated to a concentration of ∼24 mM in both cultures in the absence of headspace purging compared to levels of 3 and 6 mM in the purged Sf‐9 and High Five cultures, respectively. Headspace purging decreased the mean population doubling times of the Sf‐9 and High Five cell lines from 38 to 22 h and from 46 to 22 h, respectively. In addition, headspace purging increased the maximum cell densities obtained in the Sf‐9 and High Five cell cultures from ∼(5–6) to ∼(8–10) × 106 cells/mL and from ∼(3–5) to ∼(8–10) × 106 cells/mL, respectively.
Details
- Title: Subtitle
- Bioreactor Headspace Purging Reduces Dissolved Carbon Dioxide Accumulation in Insect Cell Cultures and Enhances Cell Growth
- Creators
- Christine Mitchell‐LogeanDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Biotechnology progress, Vol.13(6), pp.875-877
- Publisher
- American Chemical Society; USA
- DOI
- 10.1021/bp970078s
- ISSN
- 8756-7938
- eISSN
- 1520-6033
- Number of pages
- 3
- Language
- English
- Date published
- 1997
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003912002771
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