Journal article
Biosynthesis of dystroglycan: processing of a precursor propeptide
FEBS letters, Vol.468(1), pp.79-83
2000
DOI: 10.1016/S0014-5793(00)01195-9
PMID: 10683445
Abstract
Dystroglycan is a cytoskeleton-linked extracellular matrix receptor expressed in many cell types. Dystroglycan is composed of α- and β-subunits which are encoded by a single mRNA. Using a heterologous mammalian expression system, we provide the first biochemical evidence of the α/β-dystroglycan precursor propeptide prior to enzymatic cleavage. This 160 kDa dystroglycan propeptide is processed into α- and β-dystroglycan (120 kDa and 43 kDa, respectively). We also demonstrate that the precursor propeptide is glycosylated and that blockade of asparagine-linked (
N-linked) glycosylation did not prevent the cleavage of the dystroglycan precursor peptide. However, inhibition of
N-linked glycosylation results in aberrant trafficking of the α- and β-dystroglycan subunits to the plasma membrane. Thus, dystroglycan is synthesized as a precursor propeptide that is post-translationally cleaved and differentially glycosylated to yield α- and β-dystroglycan.
Details
- Title: Subtitle
- Biosynthesis of dystroglycan: processing of a precursor propeptide
- Creators
- Kathleen H Holt - Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USARachelle H Crosbie - Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USADavid P Venzke - Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USAKevin P Campbell - Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.468(1), pp.79-83
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0014-5793(00)01195-9
- PMID
- 10683445
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Language
- English
- Date published
- 2000
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984020997002771
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