Biotinylated biodegradable nanotemplated hydrogel networks for cell interactive applications

Jason D Clapper; Megan E Pearce; C Allan Guymon; Aliasger K Salem

doi:10.1021/bm701176j

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Biotinylated biodegradable nanotemplated hydrogel networks for cell interactive applications

Journal article

Peer reviewed

Biotinylated biodegradable nanotemplated hydrogel networks for cell interactive applications

Jason D Clapper, Megan E Pearce, C Allan Guymon and Aliasger K Salem

Biomacromolecules, Vol.9(4), pp.1188-1194

04/2008

DOI: 10.1021/bm701176j

PMID: 18307307

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Abstract

We describe the synthesis of a novel biotinylated nanotextured degradable hydrogel that can be rapidly surface engineered with a diverse range of biotinylated moieties. The hydrogel is synthesized by reacting methacrylated biotin-PEG with dimethacrylated P LA-b- PEG-b-P LA (LPLDMA, PEG = poly(ethylene glycol), PLA = poly(lactic acid)),or dimethacrylated PEG-b-P LA-b- PEG (PLPDMA). Methacrylated biotin-PEG is prepared by reacting biotin-PEG-OH with methacrylic anhydride. Biotin-PEG-OH is prepared by reacting alpha-hydroxy-omega-amine PEG with N-hydroxysuccinimide-biotin. Confirmation of the final product is determined using (1)H NMR and Fourier transform infrared spectroscopy (FTIR). The integrity and surface presentation of the biotin units is observed spectrophotometrically using the HABA/avidin assay. To produce nanostructured polymer topography, a self-assembling lyotropic liquid crystalline mesophase is used as a polymerization template, generating biotinylated hydrogels with highly organized lamellar matrix geometry. Traditionally processed isotropic hydrogels are used for comparison. Scanning electron microscopy shows that isotropic hydrogels have a smooth glassy appearance while lamellar templated hydrogels have defined surface topographical features that enhance preosteoblast human palatal mesenchymal cell (HEPM) attachment. Engineering the surfaces of the hydrogels with cell adhesive Arg-Gly-Asp (RGD) peptide sequences using the biotin-avidin interaction significantly enhances cell attachment. Surface engineering of cell adhesive peptides in conjunction with the lamellar template induced surface topography generates additive enhancements in cell attachment.

Biocompatible Materials - metabolism

Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry

Biocompatible Materials - chemistry

Humans

Polyethylene Glycols - chemistry

Mesoderm - cytology

Methacrylates - chemistry

Nanostructures

Spectroscopy, Fourier Transform Infrared

Avidin - metabolism

Biocompatible Materials - chemical synthesis

Biotin - chemistry

Cells, Cultured - ultrastructure

Surface Properties

Biotin - metabolism

Palate - cytology

Lactates - chemistry

Palate - metabolism

Biotinylation

Magnetic Resonance Spectroscopy - methods

Polymers - chemical synthesis

Oligopeptides - metabolism

Cell Adhesion

Polymers - chemistry

Hydrogel, Polyethylene Glycol Dimethacrylate - chemical synthesis

Mesoderm - metabolism

Polymers - metabolism

Details

Title: Subtitle: Biotinylated biodegradable nanotemplated hydrogel networks for cell interactive applications
Creators: Jason D Clapper - Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, Iowa 52242, USA
Megan E Pearce
C Allan Guymon
Aliasger K Salem
Resource Type: Journal article
Publication Details: Biomacromolecules, Vol.9(4), pp.1188-1194
DOI: 10.1021/bm701176j
PMID: 18307307
NLM abbreviation: Biomacromolecules
ISSN: 1525-7797
eISSN: 1526-4602
Publisher: United States
Language: English
Date published: 04/2008
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9983986429202771

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