Journal article
Biotransformation of 2,4-dinitroanisole by a fungal Penicillium sp
Biodegradation (Dordrecht), Vol.28(1), pp.95-109
02/2017
DOI: 10.1007/s10532-016-9780-7
PMCID: PMC5772970
PMID: 27913891
Abstract
Insensitive munitions explosives are new formulations that are less prone to unintended detonation compared to traditional explosives. While these formulations have safety benefits, the individual constituents, such as 2,4-dinitroanisole (DNAN), have an unknown ecosystem fate with potentially toxic impacts to flora and fauna exposed to DNAN and/or its metabolites. Fungi may be useful in remediation and have been shown to degrade traditional nitroaromatic explosives, such as 2,4,6-trinitrotoluene and 2,4-dinitrotoluene, that are structurally similar to DNAN. In this study, a fungal Penicillium sp., isolated from willow trees and designated strain KH1, was shown to degrade DNAN in solution within 14 days. Stable-isotope labeled DNAN and an untargeted metabolomics approach were used to discover 13 novel transformation products. Penicillium sp. KH1 produced DNAN metabolites resulting from ortho- and para-nitroreduction, demethylation, acetylation, hydroxylation, malonylation, and sulfation. Incubations with intermediate metabolites such as 2-amino-4-nitroanisole and 4-amino-2-nitroanisole as the primary substrates confirmed putative metabolite isomerism and pathways. No ring-cleavage products were observed, consistent with other reports that mineralization of DNAN is an uncommon metabolic outcome. The production of metabolites with unknown persistence and toxicity suggests further study will be needed to implement remediation with Penicillium sp. KH1. To our knowledge, this is the first report on the biotransformation of DNAN by a fungus.
Details
- Title: Subtitle
- Biotransformation of 2,4-dinitroanisole by a fungal Penicillium sp
- Creators
- Hunter W Schroer - Civil & Environmental Engineering, The University of Iowa, Iowa City, IA, 52242, USAKathryn L Langenfeld - Civil & Environmental Engineering, The University of Iowa, Iowa City, IA, 52242, USAXueshu Li - Occupational & Environmental Health, The University of Iowa, Iowa City, IA, 52242, USAHans-Joachim Lehmler - Occupational & Environmental Health, The University of Iowa, Iowa City, IA, 52242, USACraig L Just - Civil & Environmental Engineering, The University of Iowa, Iowa City, IA, 52242, USA. craig-just@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Biodegradation (Dordrecht), Vol.28(1), pp.95-109
- DOI
- 10.1007/s10532-016-9780-7
- PMID
- 27913891
- PMCID
- PMC5772970
- NLM abbreviation
- Biodegradation
- ISSN
- 0923-9820
- eISSN
- 1572-9729
- Publisher
- Netherlands
- Grant note
- P42 ES013661 / NIEHS NIH HHS P30 ES005605 / NIEHS NIH HHS
- Language
- English
- Date published
- 02/2017
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; IIHR--Hydroscience and Engineering; Iowa Superfund Research Program
- Record Identifier
- 9984001090102771
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