Journal article
Biotyping of coagulase-negative staphylococci: 108 Isolates from nosocomial bloodstream infections
Diagnostic microbiology and infectious disease, Vol.13(6), pp.461-466
1990
DOI: 10.1016/0732-8893(90)90077-9
PMID: 2279378
Abstract
Using simple, readily available typing methods, we evaluated 108 strains of coagulase-negative staphylococci that were causally related to bloodstream infections: 95% (103 of 108) of the isolates were identified as
Staphylococcus epidermidis and were divided into 18 biotypes by API Staph-Trac. A single biotype (biotype A, 6606113) accounted for >50% of the isolates of
S. epidermidis. Biotype A was further divided into seven subtypes by slime production and synergistic hemolysis; however, 66% of the isolates in biotype A remained in two major subtypes, 1a (strongly slime-positive and synergistic hemolysis-positive) and 2a (strongly slime-positive and synergistic hemolysis-negative). The addition of the antibiotype further separated the isolates into individual strains or into small groups of organisms. A significant correlation was noted between synergistic hemolysis and the three most resistant antibiotypes (
p = 4.2 × 10
-5; OR = 5.8; CI
95, 2.2-15.2). Each biotype, subtype, and antibiotype was further divided into multiple unique strains by plasmid pattern analysis. In most clinical situations the combination of API Staph-Trac, antibiotic profile, slime production, and synergistic hemolysis provides adequate strain discrimination. Plasmid pattern analysis adds important information in specific clinical situations and may be invaluable for epidemiologic investigations.
Details
- Title: Subtitle
- Biotyping of coagulase-negative staphylococci: 108 Isolates from nosocomial bloodstream infections
- Creators
- Loreen A HerwaldtLinda D BoykenMichael A Pfaller
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.13(6), pp.461-466
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0732-8893(90)90077-9
- PMID
- 2279378
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1990
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9984094747502771
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