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Bispecific BCMA/CD24 CAR-T cells control multiple myeloma growth
Journal article   Open access   Peer reviewed

Bispecific BCMA/CD24 CAR-T cells control multiple myeloma growth

Fumou Sun, Yan Cheng, Visanu Wanchai, Wancheng Guo, David Mery, Hongwei Xu, Dongzheng Gai, Eric Siegel, Clyde Bailey, Cody Ashby, …
Nature communications, Vol.15(1), 615
01/19/2024
DOI: 10.1038/s41467-024-44873-4
PMCID: PMC10798961
PMID: 38242888
url
https://doi.org/10.1038/s41467-024-44873-4View
Published (Version of record) Open Access

Abstract

Anti-multiple myeloma B cell maturation antigen (BCMA)-specific chimeric antigen receptor (CAR) T-cell therapies represent a promising treatment strategy with high response rates in myeloma. However, durable cures following anti-BCMA CAR-T cell treatment of myeloma are rare. One potential reason is that a small subset of minimal residual myeloma cells seeds relapse. Residual myeloma cells following BCMA-CAR-T-mediated treatment show less-differentiated features and express stem-like genes, including CD24. CD24-positive myeloma cells represent a large fraction of residual myeloma cells after BCMA-CAR-T therapy. In this work, we develop CD24-CAR-T cells and test their ability to eliminate myeloma cells. We find that CD24-CAR-T cells block the CD24-Siglec-10 pathway, thereby enhancing macrophage phagocytic clearance of myeloma cells. Additionally, CD24-CAR-T cells polarize macrophages to a M1-like phenotype. A dual-targeted BCMA-CD24-CAR-T exhibits improved efficacy compared to monospecific BCMA-CAR-T-cell therapy. This work presents an immunotherapeutic approach that targets myeloma cells and promotes tumor cell clearance by macrophages.
Antibodies B-Cell Maturation Antigen - genetics CD24 Antigen Humans Multiple Myeloma - pathology Neoplasm Recurrence, Local Receptors, Chimeric Antigen T-Lymphocytes

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