Bispecific monoclonal antibody complexes bound to primate erythrocyte complement receptor 1 facilitate virus clearance in a monkey model

Ronald P Taylor; William M Sutherland; Edward N Martin; Polly J Ferguson; Michele L Reinagel; Erin Gilbert; Katharine Lopez; Nino L Incardona; Hans D Ochs

doi:10.4049/jimmunol.158.2.842

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Bispecific monoclonal antibody complexes bound to primate erythrocyte complement receptor 1 facilitate virus clearance in a monkey model

Journal article

Peer reviewed

Bispecific monoclonal antibody complexes bound to primate erythrocyte complement receptor 1 facilitate virus clearance in a monkey model

Ronald P Taylor, William M Sutherland, Edward N Martin, Polly J Ferguson, Michele L Reinagel, Erin Gilbert, Katharine Lopez, Nino L Incardona and Hans D Ochs

The Journal of immunology (1950), Vol.158(2), pp.842-850

01/15/1997

DOI: 10.4049/jimmunol.158.2.842

PMID: 8993002

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Abstract

We investigated the feasibility of using bispecific mAb complexes to redirect and improve the efficiency of the primate E complement receptor 1-based clearance reaction to remove a virus from the circulation. As an initial approach, we used bacteriophage phiX174 as an immunologic model for mammalian viruses. Bispecific complexes were prepared by chemically cross-linking a mAb specific for complement receptor 1 with a mAb specific for the bacteriophage phiX174. In a monkey model these complexes facilitate rapid and quantitative binding of the target bacteriophage to E in vitro and in vivo. Moreover, after in vivo binding to E, the complexes containing mAb and prototype virus are rapidly cleared from the circulation of rhesus and cynomolgus monkeys without loss of E. Our findings suggest that bispecific mAb complexes, in concert with primate E complement receptor 1, may have therapeutic utility in the treatment of diseases associated with blood-borne pathogens.

Erythrocytes - immunology

Bacteriophage phi X 174 - immunology

Viruses - metabolism

Male

Viruses - immunology

Macaca mulatta

Antibodies, Bispecific - immunology

Receptors, Complement - metabolism

Animals

Antibodies, Bispecific - metabolism

Receptors, Complement - immunology

Erythrocytes - virology

Antigen-Antibody Complex - immunology

Disease Models, Animal

Details

Title: Subtitle: Bispecific monoclonal antibody complexes bound to primate erythrocyte complement receptor 1 facilitate virus clearance in a monkey model
Creators: Ronald P Taylor - University of Iowa, Pharmacy Practice and Science
William M Sutherland
Edward N Martin - University of Virginia
Polly J Ferguson - University of Iowa, Stead Family Department of Pediatrics
Michele L Reinagel
Erin Gilbert
Katharine Lopez
Nino L Incardona
Hans D Ochs
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.158(2), pp.842-850
DOI: 10.4049/jimmunol.158.2.842
PMID: 8993002
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AR43307 / NIAMS NIH HHS
Language: English
Date published: 01/15/1997
Academic Unit: Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Rheumatology, Allergy, and Immunology
Record Identifier: 9984071724702771

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