Black Carbon Impacts on Paraburkholderia xenovorans Strain LB400 Cell Enrichment and Activity: Implications toward Lower-Chlorinated Polychlorinated Biphenyls Biodegradation Potential

Qin Dong; Gregory H LeFevre; Timothy E Mattes

doi:10.1021/acs.est.3c09183

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Black Carbon Impacts on Paraburkholderia xenovorans Strain LB400 Cell Enrichment and Activity: Implications toward Lower-Chlorinated Polychlorinated Biphenyls Biodegradation Potential

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Black Carbon Impacts on Paraburkholderia xenovorans Strain LB400 Cell Enrichment and Activity: Implications toward Lower-Chlorinated Polychlorinated Biphenyls Biodegradation Potential

Qin Dong, Gregory H LeFevre and Timothy E Mattes

Environmental science & technology, Vol.58(8), pp.3895-3907

02/27/2024

DOI: 10.1021/acs.est.3c09183

PMCID: PMC10902836

PMID: 38356175

Appears in UI Libraries Support Open Access

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Abstract

Volatilization of lower-chlorinated polychlorinated biphenyls (LC-PCBs) from sediment poses health threats to nearby communities and ecosystems. Biodegradation combined with black carbon (BC) materials is an emerging bioaugmentation approach to remove PCBs from sediment, but development of aerobic biofilms on BC for long-term, sustained LC-PCBs remediation is poorly understood. This work aimed to characterize the cell enrichment and activity of biphenyl- and benzoate-grown strain LB400 on various BCs. Biphenyl dioxygenase gene ( ) abundance on four BC types demonstrated corn kernel biochar hosted at least 4 orders of magnitude more attached cells per gram than other feedstocks, and microscopic imaging revealed the attached live cell fraction was >1.5× more on corn kernel biochar than GAC. BC characteristics (i.e., sorption potential, pore size, pH) appear to contribute to cell attachment differences. Reverse transcription qPCR indicated that BC feedstocks significantly influenced expression in attached cells. The transcript-per-gene ratio of attached cells was >10-fold more than suspended cells, confirmed by transcriptomics. RNA-seq also demonstrated significant upregulation of biphenyl and benzoate degradation pathways on attached cells, as well as revealing biofilm formation potential/cell-cell communication pathways. These novel findings demonstrate aerobic PCB-degrading cell abundance and activity could be tuned by adjusting BC feedstocks/attributes to improve LC-PCBs biodegradation potential.

Biofilms

corn kernel biochar

GAC

Paraburkholderia xenovorans strain LB400

biphenyl dioxygenase gene expression

UIOWA OA Agreement

Details

Title: Subtitle: Black Carbon Impacts on Paraburkholderia xenovorans Strain LB400 Cell Enrichment and Activity: Implications toward Lower-Chlorinated Polychlorinated Biphenyls Biodegradation Potential
Creators: Qin Dong - IIHR─Hydroscience and Engineering, University of Iowa, 100 C. Maxwell Stanley Hydraulics Laboratory, Iowa City, Iowa 52242, United States
Gregory H LeFevre - IIHR─Hydroscience and Engineering, University of Iowa, 100 C. Maxwell Stanley Hydraulics Laboratory, Iowa City, Iowa 52242, United States
Timothy E Mattes - University of Iowa
Resource Type: Journal article
Publication Details: Environmental science & technology, Vol.58(8), pp.3895-3907
DOI: 10.1021/acs.est.3c09183
PMID: 38356175
PMCID: PMC10902836
NLM abbreviation: Environ Sci Technol
eISSN: 1520-5851
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: NIH P42ES013661
Language: English
Electronic publication date: 02/14/2024
Date published: 02/27/2024
Academic Unit: Civil and Environmental Engineering; Iowa Superfund Research Program
Record Identifier: 9984559812202771

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