Blockade of Opioid Receptors in the Medullary Reticularis Nucleus Dorsalis, but not the Rostral Ventromedial Medulla, Prevents Analgesia Produced by Diffuse Noxious Inhibitory Control in Rats With Muscle Inflammation

Marcos A de Resende; Luis Felipe S Silva; Karina Sato; Lars Arendt-Nielsen; Kathleen A Sluka

doi:10.1016/j.jpain.2010.12.009

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Blockade of Opioid Receptors in the Medullary Reticularis Nucleus Dorsalis, but not the Rostral Ventromedial Medulla, Prevents Analgesia Produced by Diffuse Noxious Inhibitory Control in Rats With Muscle Inflammation

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Blockade of Opioid Receptors in the Medullary Reticularis Nucleus Dorsalis, but not the Rostral Ventromedial Medulla, Prevents Analgesia Produced by Diffuse Noxious Inhibitory Control in Rats With Muscle Inflammation

Marcos A de Resende, Luis Felipe S Silva, Karina Sato, Lars Arendt-Nielsen and Kathleen A Sluka

The journal of pain, Vol.12(6), pp.687-697

2011

DOI: 10.1016/j.jpain.2010.12.009

PMCID: PMC3954524

PMID: 21330219

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Abstract

Diffuse Noxious Inhibitory Controls (DNIC) involves application of a noxious stimulus outside the testing site to produce analgesia. In human subjects with a variety of chronic pain conditions, DNIC is less effective; however, in animal studies, DNIC is more effective after tissue injury. While opioids are involved in DNIC analgesia, the pathways involved in this opioid-induced analgesia are not clear. The aim of the present study was to test the effectiveness of DNIC in inflammatory muscle pain, and to study which brainstem sites mediate DNIC- analgesia. Rats were injected with 3% carrageenan into their gastrocnemius muscle and responses to cutaneous and muscle stimuli were assessed before and after inflammation, and before and after DNIC induced by noxious heat applied to the tail (45°C and 47°C). Naloxone was administered systemically, into rostral ventromedial medulla (RVM), or bilaterally into the medullary reticularis nucleus dorsalis (MdD) prior to the DNIC-conditioning stimuli. DNIC produced a similar analgesic effect in both acute and the chronic phases of inflammation reducing both cutaneous and muscle sensitivity in a dose-dependent manner. Naloxone systemically or microinjected into the MdD prevented DNIC-analgesia, while naloxone into the RVM had no effect on DNIC analgesia. Thus, DNIC analgesia involves activation of opioid receptors in the MdD. The current study shows that DNIC activates opioid receptors in the MdD, but not the RVM, to produce analgesia. These data are important for understanding clinical studies on DNIC as well as for potential treatment of chronic pain patients.

inhibition

Pain

DNIC

muscle

opioid

Details

Title: Subtitle: Blockade of Opioid Receptors in the Medullary Reticularis Nucleus Dorsalis, but not the Rostral Ventromedial Medulla, Prevents Analgesia Produced by Diffuse Noxious Inhibitory Control in Rats With Muscle Inflammation
Creators: Marcos A de Resende - Rehabilitation Science Program, Federal University of Minas Gerais, Brazil
Luis Felipe S Silva - Physical Therapy and Rehabilitation Science, College of Medicine, University of Iowa, Iowa City, Iowa
Karina Sato - Physical Therapy and Rehabilitation Science, College of Medicine, University of Iowa, Iowa City, Iowa
Lars Arendt-Nielsen - Center for Sensory-Motor Interactions (SMI), Department of Health Science and Technology, Aalborg University, Denmark
Kathleen A Sluka - Physical Therapy and Rehabilitation Science, College of Medicine, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: The journal of pain, Vol.12(6), pp.687-697
DOI: 10.1016/j.jpain.2010.12.009
PMID: 21330219
PMCID: PMC3954524
NLM abbreviation: J Pain
ISSN: 1526-5900
eISSN: 1528-8447
Publisher: Elsevier Inc
Grant note: AR052316 / National Institutes of Health
Language: English
Date published: 2011
Academic Unit: Iowa Neuroscience Institute; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
Record Identifier: 9984040011602771

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