Journal article
Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins
Journal of dentistry, Vol.52, pp.79-86
09/01/2016
DOI: 10.1016/j.jdent.2016.07.016
PMID: 27472956
Abstract
Objectives: This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)).
Methods: Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4-3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (mu TBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis.
Results: Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable mTBS over 6 months. Conversely, mTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis).
Significance: The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface. (C) 2016 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins
- Creators
- Gabriel Abuna - Universidade Estadual de Campinas (UNICAMP)Victor P. Feitosa - Universidade Federal do CearáAmerico Bortolazzo Correr - Universidade Estadual de Campinas (UNICAMP)Giuseppe Cama - Ghent UniversityMarcelo Giannini - Universidade Estadual de Campinas (UNICAMP)Mario A. Sinhoreti - Universidade Estadual de Campinas (UNICAMP)David H. Pashley - Augusta UniversitySalvatore Sauro - Universidad Cardenal Herrera CEU
- Resource Type
- Journal article
- Publication Details
- Journal of dentistry, Vol.52, pp.79-86
- Publisher
- Elsevier
- DOI
- 10.1016/j.jdent.2016.07.016
- PMID
- 27472956
- ISSN
- 0300-5712
- eISSN
- 1879-176X
- Number of pages
- 8
- Grant note
- PEST-1408; INDI1527 / Programa de Consolidacion de Indicadores: Fomento Plan Estatal CEU-UCH
- Language
- English
- Date published
- 09/01/2016
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984654468402771
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