Journal article
Bone Cell Expression on Titanium Surfaces is Altered by Sterilization Treatments
Journal of dental research, Vol.73(5), pp.1061-1071
05/1994
DOI: 10.1177/00220345940730050801
PMID: 8006233
Abstract
Phenotypic responses of rat calvarial osteoblast-like cells (RCOB) were evaluated on commercially pure titanium (cpTi) surfaces when cultured at high density (5100 cells/mm2). These surfaces were prepared to three different clinically relevant surface preparations (1-μm, 600-grit, and 50-μm-grit sand-blast), followed by sterilization with either ultraviolet light, ethylene oxide, argon plasma-cleaning, or routine clinical autoclaving. Osteocalcin and alkaline phosphatase, but not collagen expression, were significantly affected by surface roughness when these surfaces were altered by argon plasma-cleaning. In general, plasma-cleaned cpTi surfaces demonstrated an inverse relationship between surface roughness and phenotypic markers for a bone-like response. On a per-cell basis, levels of the bone-specific protein, osteocalcin, and the enzymatic activity of alkaline phosphatase were highest on the smooth 1-μm polished surface and lowest on the roughest surfaces for the plasma-cleaned cpTi. Detectable bone cell expression can be altered by clinically relevant surfaces prepared by standard dental implant preparation techniques.
Details
- Title: Subtitle
- Bone Cell Expression on Titanium Surfaces is Altered by Sterilization Treatments
- Creators
- C.M Stanford - Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, Iowa 52242J.C Keller - Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, Iowa 52242M Solursh - Department of Biological Sciences, N405 Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.73(5), pp.1061-1071
- DOI
- 10.1177/00220345940730050801
- PMID
- 8006233
- NLM abbreviation
- J Dent Res
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Publisher
- SAGE Publications; Los Angeles, CA
- Language
- English
- Date published
- 05/1994
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Graduate College Admin and Gen; President; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
- Record Identifier
- 9984065811002771
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