Journal article
Bone Marrow-Derived Mesenchymal Stromal Cells (MSCs) Modulate the Inflammatory Character of Alveolar Macrophages from Sarcoidosis Patients
Journal of clinical medicine, Vol.9(1), p.278
01/19/2020
DOI: 10.3390/jcm9010278
PMCID: PMC7019909
PMID: 31963936
Abstract
Sarcoidosis is a devastating inflammatory disease affecting many organs, especially the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) can “reprogram” various types of macrophages towards an anti-inflammatory phenotype. We wanted to determine whether alveolar macrophages from sarcoidosis subjects behave similarly by mounting an anti-inflammatory response when co-cultured with MSCs. Fifteen sarcoidosis and eight control subjects underwent bronchoscopy and bronchoalveolar lavage (BAL). Unselected BAL cells (70–94% macrophages) were isolated and cultured with and without MSCs from healthy adults. Following stimulation of the cultured cells with lipopolysaccharide, the medium was removed to measure interleukin 10 and tumor necrosis factor alpha (IL-10 and TNF-α). In two additional sarcoidosis subjects, flow cytometry was used to study intracellular cytokines and surface markers associated with alveolar macrophages to confirm the results. Unselected BAL cells from sarcoidosis subjects co-cultured with MSCs showed a reduction in TNF-α (pro-inflammatory M1) and an increase in IL-10 (anti-inflammatory M2) in 9 of 11 samples studied. Control subject samples showed few, if any, differences in cytokine production. Unselected BAL cells from two additional patients analyzed by flow cytometry confirmed a switch towards an anti-inflammatory state (i.e., M1 to M2) after co-culture with MSCs. These results suggest that, similarly to other macrophages, alveolar macrophages also respond to MSC contacts by changing towards an anti-inflammatory phenotype. Based on our results, we hypothesize that mesenchymal stromal cells applied to the airways might alleviate lung inflammation and decrease steroid need in patients with sarcoidosis.
Details
- Title: Subtitle
- Bone Marrow-Derived Mesenchymal Stromal Cells (MSCs) Modulate the Inflammatory Character of Alveolar Macrophages from Sarcoidosis Patients
- Creators
- Ian McClain CaldwellChristopher Hogden - National Institute of Dental and Craniofacial ResearchKrisztian NemethMichael Boyajian - National Institute of Dental and Craniofacial ResearchMiklos Krepuska - National Institute of Dental and Craniofacial ResearchGergely Szombath - Semmelweis UniversitySandra MacDonald - National Heart Lung and Blood InstituteMehrnoosh Abshari - National Institute of Dental and Craniofacial ResearchJoel Moss - National Heart Lung and Blood InstituteLynn Vitale-Cross - National Institute of Dental and Craniofacial ResearchJoseph R FontanaEva Mezey - National Institute of Dental and Craniofacial Research
- Resource Type
- Journal article
- Publication Details
- Journal of clinical medicine, Vol.9(1), p.278
- DOI
- 10.3390/jcm9010278
- PMID
- 31963936
- PMCID
- PMC7019909
- NLM abbreviation
- J Clin Med
- ISSN
- 2077-0383
- eISSN
- 2077-0383
- Publisher
- MDPI
- Grant note
- DOI: 10.13039/100000009, name: National Institutes of Health, award: Intramural Research Program
- Language
- English
- Date published
- 01/19/2020
- Academic Unit
- Endodontics
- Record Identifier
- 9984367737902771
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