Journal article
Bone Marrow Stromal Cells Promote Neurite Extension in Organotypic Spinal Cord Slice: Significance for Cell Transplantation Therapy
Neurorehabilitation and neural repair, Vol.22(5), pp.447-457
09/2008
DOI: 10.1177/1545968308315596
PMID: 18780880
Abstract
Objective. Recent reports have indicated that bone marrow stromal cells (BMSCs) have the potential to improve neurological function when transplanted into models of central nervous system (CNS) disorders, including traumatic spinal cord injury. In this study, the authors aimed to clarify the underlying mechanism through which BMSCs supported CNS regeneration in the spinal cord. Methods. The authors topically applied mouse BMSCs expressing green fluorescence protein (0.4-4 × 104 cells) on the organotypic spinal cord slice culture prepared from 6-day-old rat pups (n = 17). They were co-cultured for 3 weeks after the slice culture started, and the behavior of the applied BMSCs was serially observed using a fluorescence bioimaging technique. The authors completed a histological analysis at the end of the co-cultures and evaluated the profiles of the cultured BMSCs using microarray and immunocytochemistry techniques. Results. The fluorescence bioimaging showed that the BMSCs survived and made a cluster on the slice during the experiments. They also induced a morphological change in the slice within 48 hours of co-culture. Immunohistochemistry analysis showed that the BMSCs promoted a marked neurite extension toward their cluster and some of the BMSCs expressed Tuj-1, an early neuronal marker. Analysis by microarray and immunocytochemistry revealed that BMSCs highly expressed the matrix metalloproteinases (MMPs), stromal cell—derived factor-1, and its specific receptor CXCR4. Conclusions . These findings suggest that the donor BMSCs can support CNS regeneration due to their acquisition of a suitable environment for differentiation and promotion of neurite extension via MMPs and chemokines.
Details
- Title: Subtitle
- Bone Marrow Stromal Cells Promote Neurite Extension in Organotypic Spinal Cord Slice: Significance for Cell Transplantation Therapy
- Creators
- Hideo Shichinohe - Department of Neurosurgery Hokkaido University Graduate School of MedicineSatoshi Kuroda - Department of Neurosurgery Hokkaido University Graduate School of MedicineSachiko Tsuji - Department of Neurology Hokkaido University Graduate School of Medicine, Hokkaido, JapanSatoshi Yamaguchi - Department of Neurosurgery Hokkaido University Graduate School of MedicineShunsuke Yano - Department of Neurosurgery Hokkaido University Graduate School of MedicineJang-Bo Lee - Department of Neurosurgery Hokkaido University Graduate School of MedicineHiroyuki Kobayashi - Department of Neurosurgery Hokkaido University Graduate School of MedicineSeiji Kikuchi - Department of Neurology Hokkaido University Graduate School of Medicine, Hokkaido, JapanKazutoshi Hida - Department of Neurosurgery Hokkaido University Graduate School of MedicineYoshinobu Iwasaki - Department of Neurosurgery Hokkaido University Graduate School of Medicine
- Resource Type
- Journal article
- Publication Details
- Neurorehabilitation and neural repair, Vol.22(5), pp.447-457
- Publisher
- SAGE Publications; Los Angeles, CA
- DOI
- 10.1177/1545968308315596
- PMID
- 18780880
- ISSN
- 1545-9683
- eISSN
- 1552-6844
- Language
- English
- Date published
- 09/2008
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984040345302771
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