Journal article
Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-β1
Journal of biomaterials science. Polymer ed, Vol.11(8), pp.891-901
01/01/2000
DOI: 10.1163/156856200744084
Abstract
The objective of the present study is to investigate the addition effect of transforming growth factor (TGF)-β1 on the bone formation at a rabbit skull defect induced by autologous bone marrow (BM).
Following application of gelatin microspheres containing TGF-β1, with or without BM cells to skull bone defects, bone formation at the defect was assessed by soft X-ray, dual energy X-ray absorptometry
(DEXA), and histological examinations. After implantation for 6 weeks, gelatin microspheres containing 0.05 μg of TGF-β1 plus 10
6
of BM cells induced bone formation at the 6 mm diameter
bone defect. The defect was histologically closed by newly formed bone tissue, whilst both gelatin microspheres containing 0.05 μg of TGF-β1, and 10
6
and 10
7
of BM cells were
ineffective. A DEXA experiment revealed that combination of gelatin microspheres containing TGF-β1 with BM cells enhanced the bone mineral density at the skull defect to a significantly greater extent
than other agents. These findings indicate that a combination of gelatin microspheres containing TGF-β1 enabled BM cells to enhance the osteoinductive ability, resulting in bone formation even at the
cell number at which BM cells alone were ineffective.
Details
- Title: Subtitle
- Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-β1
- Creators
- Yasuhiko Tabata - Kyoto UniversityLiu HongSusumu MiyamotoManabu MiyaoNobuo HashimotoYoshito Ikada
- Resource Type
- Journal article
- Publication Details
- Journal of biomaterials science. Polymer ed, Vol.11(8), pp.891-901
- DOI
- 10.1163/156856200744084
- ISSN
- 0920-5063
- eISSN
- 1568-5624
- Publisher
- Taylor & Francis Group
- Language
- English
- Date published
- 01/01/2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367646202771
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