Bone marrow cells produce a novel TSHβ splice variant that is upregulated in the thyroid following systemic virus infection

Bryce H. Vincent; Dina Montufar-Solis; Ba-Bie Teng; Brad A. Amendt; Jeremy Schaefer; John R. Klein

doi:10.1038/gene.2008.69

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Bone marrow cells produce a novel TSHβ splice variant that is upregulated in the thyroid following systemic virus infection

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Bone marrow cells produce a novel TSHβ splice variant that is upregulated in the thyroid following systemic virus infection

Bryce H. Vincent, Dina Montufar-Solis, Ba-Bie Teng, Brad A. Amendt, Jeremy Schaefer and John R. Klein

Genes and immunity, Vol.10(1), pp.18-26

08/28/2008

DOI: 10.1038/gene.2008.69

PMCID: PMC2629504

PMID: 18754015

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Abstract

Although cells of the immune system can produce thyroid stimulating hormone (TSH), the significance of that remains unclear. Using 5′ rapid amplification of cDNA ends, we show that mouse bone marrow cells produce a novel in-frame TSHβ splice variant generated from a portion of intron 4 with all of the coding region of exon 5 but none of exon 4. The TSHβ splice variant gene was expressed at low levels in the pituitary but at high levels in the bone marrow and the thyroid, and the protein was secreted from transfected CHO cells. Immunoprecipitation identified an 8 kDa product in lysates of CHO cells transfected with the novel TSHβ construct, and a 17 kDa product in lysates of CHO cells transfected with the native TSHβ construct. The splice variant TSHβ protein elicited a cAMP response from FRTL-5 thyroid follicular cells and a mouse alveolar macrophage cell line. Expression of the TSHβ splice variant but not the native form of TSHβ was significantly upregulated in the thyroid during systemic virus infection. These studies characterize the first functional splice variant of TSHβ, which may contribute to the metabolic regulation during immunological stress, and may offer an new perspective for understanding autoimmune thyroiditis.

5′ RACE analysis

autoimmunity

isoform

metabolism

thyrotropin

Details

Title: Subtitle: Bone marrow cells produce a novel TSHβ splice variant that is upregulated in the thyroid following systemic virus infection
Creators: Bryce H. Vincent - University of Texas Dental Branch at Houston
Dina Montufar-Solis - Department of Diagnostic Sciences, Dental Branch, The University of Texas Health Science Center, Houston, USA
Ba-Bie Teng - Brown Foundation
Brad A. Amendt - Texas A&M Health Science Center
Jeremy Schaefer - Department of Diagnostic Sciences, Dental Branch, The University of Texas Health Science Center, Houston, USA
John R. Klein - Department of Diagnostic Sciences, Dental Branch, The University of Texas Health Science Center, Houston, USA
Resource Type: Journal article
Publication Details: Genes and immunity, Vol.10(1), pp.18-26
DOI: 10.1038/gene.2008.69
PMID: 18754015
PMCID: PMC2629504
NLM abbreviation: Genes Immun
ISSN: 1466-4879
eISSN: 1476-5470
Language: English
Date published: 08/28/2008
Academic Unit: Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984284343602771

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