Bone-marrow mononuclear cells reduce neurodegeneration in hippocampal CA1 layer after transient global ischemia in rats

Alane Bernardo Ramos; Andréia Vasconcelos-Dos-Santos; Sergio Augusto Lopes de Souza; Paulo Henrique Rosado-de-Castro; Lea Mirian Barbosa da Fonseca; Bianca Gutfilen; Wagner Monteiro Cintra; Rosalia Mendez-Otero

doi:10.1016/j.brainres.2013.05.024

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Bone-marrow mononuclear cells reduce neurodegeneration in hippocampal CA1 layer after transient global ischemia in rats

Journal article

Open access

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Bone-marrow mononuclear cells reduce neurodegeneration in hippocampal CA1 layer after transient global ischemia in rats

Alane Bernardo Ramos, Andréia Vasconcelos-Dos-Santos, Sergio Augusto Lopes de Souza, Paulo Henrique Rosado-de-Castro, Lea Mirian Barbosa da Fonseca, Bianca Gutfilen, Wagner Monteiro Cintra and Rosalia Mendez-Otero

Brain research, Vol.1522, pp.1-11

07/19/2013

DOI: 10.1016/j.brainres.2013.05.024

PMID: 23721927

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Abstract

Global cerebral ischemia (GCI) results in death of the pyramidal neurons in the CA1 layer of the hippocampus. In this study we used the four-vessel occlusion (4VO) model of GCI to investigate a potential neuroprotective role of bone-marrow mononuclear cells (BMMCs) transplantation. BMMCs (3×10(7)) were injected through the carotid artery, 1 or 3 days after ischemia (DAI), and the number of cells undergoing degeneration was investigated in brains at 7 DAI. A significant decrease in the number of dying cells was observed in the treated group, compared to animals treated with saline. Biodistribution of the injected cells (1 or 3 DAI) was investigated by (99m)Technetium labeling of the BMMCs and subsequent image analysis 2h after transplantation. In addition, the presence of CellTrace(™)-labeled BMMCs was investigated in tissue sections of the hippocampal area of these transplanted animals. BMMCs treatment significantly reduced the number of FJ-C positive cells in the hippocampal CA1 layer at 7 DAI. We also observed a decrease in the number of activated microglia/macrophage (ED1-positive cells) in the BMMCs-treated group compared with the untreated group. Our data show that BMMCs are able to modulate the microglial response and reduce neurodegeneration in the CA1 layer.

Animals

Bone Marrow Cells

Bone Marrow Transplantation - methods

Brain Ischemia - pathology

CA1 Region, Hippocampal - pathology

Leukocytes, Mononuclear - transplantation

Male

Nerve Degeneration - pathology

Rats

Rats, Wistar

Details

Title: Subtitle: Bone-marrow mononuclear cells reduce neurodegeneration in hippocampal CA1 layer after transient global ischemia in rats
Creators: Alane Bernardo Ramos - Universidade Federal do Rio de Janeiro
Andréia Vasconcelos-Dos-Santos - Universidade Federal do Rio de Janeiro
Sergio Augusto Lopes de Souza
Paulo Henrique Rosado-de-Castro - National Institute of Science and Technology for Structural Biology and Bioimaging
Lea Mirian Barbosa da Fonseca
Bianca Gutfilen - Universidade Federal do Rio de Janeiro
Wagner Monteiro Cintra - Universidade Federal do Rio de Janeiro
Rosalia Mendez-Otero - Universidade Federal do Rio de Janeiro
Resource Type: Journal article
Publication Details: Brain research, Vol.1522, pp.1-11
DOI: 10.1016/j.brainres.2013.05.024
PMID: 23721927
NLM abbreviation: Brain Res
ISSN: 0006-8993
eISSN: 1872-6240
Language: English
Date published: 07/19/2013
Academic Unit: Radiology
Record Identifier: 9985143551202771

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