Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo

Philip H Jones; Harshini V Mehta; Martina Maric; Richard J Roller; Chioma M Okeoma

doi:10.1186/1742-4690-9-10

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Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo

Journal article

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Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo

Philip H Jones, Harshini V Mehta, Martina Maric, Richard J Roller and Chioma M Okeoma

Retrovirology, Vol.9(1), pp.10-10

2012

DOI: 10.1186/1742-4690-9-10

PMCID: PMC3283513

PMID: 22284121

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Abstract

Background: Bone marrow stromal cell antigen 2 (BST-2) is a cellular factor that restricts the egress of viruses such as human immunodeficiency virus (HIV-1) from the surface of infected cells, preventing infection of new cells. BST-2 is variably expressed in most cell types, and its expression is enhanced by cytokines such as type I interferon alpha (IFN-α). In this present study, we used the beta-retrovirus, mouse mammary tumor virus (MMTV) as a model to examine the role of mouse BST-2 in host infection in vivo. Results: By using RNA interference, we show that loss of BST-2 enhances MMTV replication in cultured mammary tumor cells and in vivo. In cultured cells, BST-2 inhibits virus accumulation in the culture medium, and co-localizes at the cell surface with virus structural proteins. Furthermore, both scanning electron micrograph (SEM) and transmission electron micrograph (TEM) show that MMTV accumulates on the surface of IFNα-stimulated cells. Conclusions: Our data provide evidence that BST-2 restricts MMTV release from naturally infected cells and that BST-2 is an antiviral factor in vivo.

BST-2

In vivo

Tetherin

MMTV

Interferon alpha

SEM

Research

TEM

Details

Title: Subtitle: Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo
Creators: Philip H Jones - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa City, IA, USA
Harshini V Mehta - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa City, IA, USA
Martina Maric - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa City, IA, USA
Richard J Roller - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa City, IA, USA
Chioma M Okeoma - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Retrovirology, Vol.9(1), pp.10-10
DOI: 10.1186/1742-4690-9-10
PMID: 22284121
PMCID: PMC3283513
NLM abbreviation: Retrovirology
ISSN: 1742-4690
eISSN: 1742-4690
Publisher: BioMed Central
Language: English
Date published: 2012
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001210902771

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