Journal article
Bone regeneration by transforming growth factor β1 released from a biodegradable hydrogel
Journal of controlled release, Vol.64(1), pp.133-142
2000
DOI: 10.1016/S0168-3659(99)00129-7
Abstract
This paper describes the sustained release of transforming growth factor β1 (TGF-β1) from a biodegradable hydrogel based on polyion complexation for the enhancement of bone regeneration activity. Basic TGF-β1 was adsorbed onto the biodegradable hydrogel of acidic gelatin with an isoelectric point of 5.0 by an electrostatic interaction. The TGF-β1 could not be adsorbed onto basic gelatin. When acidic gelatin hydrogels incorporating
125I-labeled TGF-β1 were implanted into the back subcutis of mice, the radioactivity decreased with time and the in vivo retention of TGF-β1 was prolonged with a decrease in the water content of hydrogels. The higher the water content of hydrogels, the faster their biodegradation. The in vivo retention of TGF-β1 correlated well with that of gelatin hydrogels, indicating that TGF-β1 was released from the gelatin hydrogel as a result of hydrogel biodegradation. The ability of TGF-β1-incorporated into acidic gelatin hydrogels to induce bone regeneration was evaluated in a rabbit calvarial defect model. Eight weeks after treatment, the gelatin hydrogels with water contents of 90 and 95 wt% induced significantly high bone regeneration compared with those with lower and higher water contents and free TGF-β1. This indicates that the sustained release of TGF-β1 from the hydrogel with suitable in vivo degradability is necessary to effectively enhance its osteoinductive function. Rapid hydrogel degradation will result in a retention time of TGF-β1 which is too short to induce bone regeneration. It is possible that the slow degradation of the hydrogel physically blocked TGF-β1-induced bone regeneration at the skull defect. It can be concluded that the gelatin hydrogel is a promising matrix of TGF-β1 release to induce skull bone regeneration.
Details
- Title: Subtitle
- Bone regeneration by transforming growth factor β1 released from a biodegradable hydrogel
- Creators
- Masaya Yamamoto - Kyoto UniversityYasuhiko Tabata - Kyoto UniversityLiu Hong - Kyoto UniversitySusumu Miyamoto - Kyoto UniversityNobuo Hashimoto - Kyoto UniversityYoshito Ikada - Kyoto University
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.64(1), pp.133-142
- DOI
- 10.1016/S0168-3659(99)00129-7
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367650402771
Metrics
14 Record Views