Journal article
Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors
ACS applied bio materials, Vol.3(9), pp.5652-5663
09/21/2020
DOI: 10.1021/acsabm.0c00371
PMID: 35021797
Abstract
Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, through their ability to serve as a carrier for potent growth factor, bone morphogenic protein-2 (BMP-2). However, biophysical properties of hydrogels such as multiscale structural hierarchy and bone extracellular matrix (ECM)-mimetic microarchitecture are underutilized while designing current bone grafts. Incorporation of these properties offers great potential to create a favorable biomimetic microenvironment to harness their regenerative potential. Here, we present our approach to fabricate collagen-inspired bioactive hydrogel scaffolds (referred to as "RegenMatrix") to guide and enhance bone regeneration in a rabbit ulna defect model through the mimicry of multiscale architecture of bone ECM, i.e., native collagen. Specifically, we employed polyelectrolyte complexation to promote bottom-up self-assembly of oppositely charged polysaccharides (chitosan and kappa-carrageenan) at multiple length scales forming fibrils, which further assemble into fibers. The self-assembly and bioinspired scaffold fabrication method resulted in robust cylindrical RegenMatrix with excellent retention of the multiscale architecture and uniform mineral deposition throughout the scaffolds. RegenMatrix, in both nonmineralized and mineralized forms, enhanced bone regeneration in the semiload-bearing ulna defect when compared to the empty defect. RegenMatrix also showed greater histocompatibility without any fibrous tissue formation. Collectively, the RegenMatrix developed in this study has a great potential as a bioactive bone graft without any added growth factors.
Details
- Title: Subtitle
- Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors
- Creators
- Akhil Patel - University of PittsburghSamer H. Zaky - University of PittsburghHongshuai Li - University of PittsburghKaren Schoedel - University of PittsburghAlejandro J. Almarza - University of PittsburghCharles Sfeir - University of PittsburghVinayak Sant - University of PittsburghShilpa Sant - University of Pittsburgh
- Resource Type
- Journal article
- Publication Details
- ACS applied bio materials, Vol.3(9), pp.5652-5663
- Publisher
- Amer Chemical Soc
- DOI
- 10.1021/acsabm.0c00371
- PMID
- 35021797
- ISSN
- 2576-6422
- eISSN
- 2576-6422
- Number of pages
- 12
- Grant note
- Henry L. Hillman Foundation P30CA047904 / National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Center for Medical Innovation, Swanson School of Engineering, University of Pittsburgh University of Pittsburgh School of Pharmacy
- Language
- English
- Date published
- 09/21/2020
- Academic Unit
- Orthopedics and Rehabilitation; Radiation Oncology
- Record Identifier
- 9984304682102771
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