Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo

G. Di Pasquale; L. Ostedgaard; D. Vermeer; W. D. Swaim; P. Karp; J. A. Chiorini

doi:10.1038/gt.2011.138

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Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo

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Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo

G. Di Pasquale, L. Ostedgaard, D. Vermeer, W. D. Swaim, P. Karp and J. A. Chiorini

Gene therapy, Vol.19(5), pp.576-581

05/01/2012

DOI: 10.1038/gt.2011.138

PMCID: PMC3266448

PMID: 22011646

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Abstract

Bovine adeno-associated virus (BAAV) can enter a cell either through a transcytosis or transduction pathway. We previously demonstrated that particles entering via the transcytosis pathway can be redirected to transduce the cell by blocking particle exocytosis with tannic acid (TA). To investigate whether this approach is useful in lung gene therapy applications, we tested the effect of TA on BAAV transduction in cystic fibrosis airway epithelia in vitro, and in mouse lung in vivo. Our findings suggest that BAAV transcytosis can occur in vivo and that treatment with TA reduces transcytosis and increases lung transduction. TA treatment did not impair the sorting and the activity of the BAAV expressed cystic fibrosis transmembrane regulator membrane protein. Gene Therapy (2012) 19, 576-581; doi:10.1038/gt.2011.138; published online 20 October 2011

Biochemistry & Molecular Biology

Biotechnology & Applied Microbiology

Genetics & Heredity

Life Sciences & Biomedicine

Medicine, Research & Experimental

Research & Experimental Medicine

Science & Technology

Details

Title: Subtitle: Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo
Creators: G. Di Pasquale - National Institute of Dental and Craniofacial Research
L. Ostedgaard - University of Iowa
D. Vermeer - University of Iowa
W. D. Swaim - National Institute of Dental and Craniofacial Research
P. Karp - University of Iowa
J. A. Chiorini - National Institute of Dental and Craniofacial Research
Resource Type: Journal article
Publication Details: Gene therapy, Vol.19(5), pp.576-581
Publisher: Springer Nature
DOI: 10.1038/gt.2011.138
PMID: 22011646
PMCID: PMC3266448
ISSN: 0969-7128
eISSN: 1476-5462
Number of pages: 6
Grant note: ZIADE000695 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) NIH/NIDCR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
Language: English
Date published: 05/01/2012
Academic Unit: Internal Medicine
Record Identifier: 9984360052802771

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