Journal article
Brain cancer stem-like cell genesis from p53-deficient mouse astrocytes by oncogenic Ras
Biochemical and biophysical research communications, Vol.365(3), pp.496-502
01/18/2008
DOI: 10.1016/j.bbrc.2007.11.005
PMID: 18021740
Abstract
Here, we show that H-ras
V12 causes the p53-knockout mouse astrocytes (p53−/− astrocytes) to be transformed into brain cancer stem-like cells. H-ras
V12 triggers the p53−/− astrocytes to express a Nestin and a Cd133, which are expressed in normal and cancer neural stem cells. H-ras
V12 also induces the formation of a single cell-derived neurosphere under neural stem cell culture conditions. Furthermore, H-ras
V12-overexpressing p53−/− astrocytes (p53−/−ast-H-ras
V12) possess an
in vitro self-renewal capacity, and are aberrantly differentiated into Tuj1-positve neurons both
in vitro and
in vivo. Amongst a variety of Ras-mediated canonical signaling pathways, we demonstrated that the MEK/ERK signaling pathway is responsible for neurosphere formation in p53-deficient astrocytes, whereas the PI3K/AKT signaling pathway is involved in oncogenic transformation in these cells. These findings suggest that the activation of Ras signaling pathways promotes the generation of brain cancer stem-like cells from p53-deficient mouse astrocytes by changing cell fate and transforming cell properties.
Details
- Title: Subtitle
- Brain cancer stem-like cell genesis from p53-deficient mouse astrocytes by oncogenic Ras
- Creators
- Joong-Seob Lee - Korea UniversityJung-Eun Gil - Korea UniversityJong-Hoon Kim - Korea UniversityTae-Kyung Kim - Korea UniversityXun Jin - Korea UniversitySe-Yeong Oh - Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of KoreaYoung-Woo Sohn - Korea UniversityHye-Min Jeon - Korea UniversityHyo-Jung Park - Korea UniversityJong-Whi Park - Korea UniversityYong-Jae Shin - Korea UniversityYong Gu Chung - Korea UniversityJang-Bo Lee - Korea UniversitySeungkwon You - Korea UniversityHyunggee Kim - Korea University
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.365(3), pp.496-502
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2007.11.005
- PMID
- 18021740
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 01/18/2008
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984459627302771
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