Journal article
Brain expression of inducible cyclooxygenase 2 messenger RNA in rats undergoing cardiopulmonary bypass
Anesthesiology, Vol.95(6), pp.1380-1388
12/2001
DOI: 10.1097/00000542-200112000-00017
PMID: 11748396
Abstract
We hypothesized that systemic proinflammatory cytokines or endotoxemia, or both, associated with cardiopulmonary bypass (CPB) would increase expression of inducible cyclooxygenase (COX-2) or inducible nitric oxide synthase (iNOS) messenger RNA (mRNA), or both, in brain. Isoflurane-anesthetized Sprague-Dawley rats were randomly selected for CPB (n = 6) or sham surgery (n = 6). All animals underwent tracheotomy and controlled ventilation, arterial and venous pressure monitoring, insertion of a jugular venous outflow catheter, insertion of a subclavian arterial inflow catheter, systemic anticoagulation (500 U/kg heparin) and, except during CPB, servoregulation of pericranial temperature at 37.5 degrees C. Animals selected for CPB underwent 1 h of CPB at 165 ml x kg(-1) x min(-1) (31.8 +/- 0.2 degrees C), whereas animals having sham surgery underwent no intervention during this interval. Thereafter, all animals were given protamine and remained anesthetized for 4 more h. Brain and liver COX-2 and iNOS mRNA expression were determined by a ribonuclease protection assay with ribosomal L32 mRNA as a loading control. Arterial blood was analyzed for interleukin 1beta, interleukin 6, and endotoxin concentrations. Endotoxin concentrations did not increase above baseline values in either group. At 4 h after the CPB interval, interleukin 6 concentrations were significantly greater in CPB animals (101 +/- 45 pg/ml) versus sham animals (44 +/- 17 pg/ml) (P = 0.025). Brain COX-2 expression was significantly greater in CPB animals (0.36 +/- 0.11) versus shams (0.19 +/- 0.08) (P = 0.013). Brain COX-2 expression correlated with interleukin 6 concentration 4 h after CPB (r = 0.91; P = 5 x 10(-5)). In brain, iNOS mRNA was not detected in any animal. Cardiopulmonary bypass animals had only trace COX-2 and iNOS mRNA induction in liver. Cardiopulmonary bypass was associated with increased systemic interleukin 6 concentrations and increased brain COX-2 expression.
Details
- Title: Subtitle
- Brain expression of inducible cyclooxygenase 2 messenger RNA in rats undergoing cardiopulmonary bypass
- Creators
- B J Hindman - Department of Anesthesia, University of Iowa, College of Medicine, Iowa City, USA. Brad-Hindman@uiowa.eduS A MooreJ CutkompT SmithS E Ross-BartaF DexterJ E Brian Jr
- Resource Type
- Journal article
- Publication Details
- Anesthesiology, Vol.95(6), pp.1380-1388
- DOI
- 10.1097/00000542-200112000-00017
- PMID
- 11748396
- NLM abbreviation
- Anesthesiology
- ISSN
- 0003-3022
- eISSN
- 1528-1175
- Publisher
- United States
- Grant note
- NS24621 / NINDS NIH HHS
- Language
- English
- Date published
- 12/2001
- Academic Unit
- Health Management and Policy; Pathology; Anesthesia
- Record Identifier
- 9983806251102771
Metrics
44 Record Views