Brain microvascular smooth muscle expresses class II antigens

Michael N Hart; Mary M Waldschmidt; Joan M Hanley-Hyde; Steven A Moore; John D Kemp; Robert L Schelper

doi:10.4049/jimmunol.138.9.2960

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Brain microvascular smooth muscle expresses class II antigens

Journal article

Peer reviewed

Brain microvascular smooth muscle expresses class II antigens

Michael N Hart, Mary M Waldschmidt, Joan M Hanley-Hyde, Steven A Moore, John D Kemp and Robert L Schelper

The Journal of immunology (1950), Vol.138(9), pp.2960-2963

1987

DOI: 10.4049/jimmunol.138.9.2960

PMID: 3471815

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Abstract

Mouse (BALB/c) splenic lymphocytes co-cultured in vitro with syngeneic brain-derived microvascular smooth muscle (SM) proliferate and become activated. After subsequent transfer of the activated lymphocytes to a syngeneic host, a vasculitis develops in the host. Investigation of the possible antigen-presenting properties of the cultured SM has resulted in the demonstration of class II (Ia) antigens on the SM. Fluorescence-activated cell sorter analysis has shown that an average of 31% of unstimulated SM cells in culture were positive when stained with an anti-IE of the appropriate haplotype (H2d), and an average of 20% were positive with an anti-IA of the H2d haplotype. Controls consisting of irrelevant antibodies of the same isotype, as well as an anti-IA of the H2s haplotype, were negative. In contrast, BALB/c-derived brain microvascular endothelial cells showed considerably less class II antigen expression (7% for both IA and IE).

Fundamental and applied biological sciences. Psychology

Fundamental immunology

Biological and medical sciences

Autoimmunity (experimental aspects and models)

Details

Title: Subtitle: Brain microvascular smooth muscle expresses class II antigens
Creators: Michael N Hart - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Mary M Waldschmidt - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Joan M Hanley-Hyde - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Steven A Moore - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
John D Kemp - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Robert L Schelper - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.138(9), pp.2960-2963
Publisher: American Association of Immunologists; Bethesda, MD
DOI: 10.4049/jimmunol.138.9.2960
PMID: 3471815
ISSN: 0022-1767
eISSN: 1550-6606
Language: English
Date published: 1987
Academic Unit: Pathology
Record Identifier: 9984047890402771

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