Journal article
Break-induced replication mechanisms in yeast and mammals
Current opinion in genetics & development, Vol.71, pp.163-170
12/2021
DOI: 10.1016/j.gde.2021.08.002
PMCID: PMC9109633
PMID: 34481360
Abstract
Break-induced replication (BIR) is a pathway specialized in repair of double-strand DNA breaks with only one end capable of invading homologous template that can arise following replication collapse, telomere erosion or DNA cutting by site-specific endonucleases. For a long time, yeast remained the only model system to study BIR. Studies in yeast demonstrated that BIR represents an unusual mode of DNA synthesis that is driven by a migrating bubble and leads to conservative inheritance of newly synthesized DNA. This unusual type of DNA synthesis leads to high levels of mutations and chromosome rearrangements. Recently, multiple examples of BIR were uncovered in mammalian cells that allowed the comparison of BIR between organisms. It appeared initially that BIR in mammalian cells is predominantly independent of RAD51, and therefore different from BIR that is predominantly Rad51-dependent in yeast. However, a series of systematic studies utilizing site-specific DNA breaks for BIR initiation in mammalian reporters led to the discovery of highly efficient RAD51-dependent BIR, allowing side-by side comparison with BIR in yeast which is the focus of this review.
Details
- Title: Subtitle
- Break-induced replication mechanisms in yeast and mammals
- Creators
- Xiaohua Wu - Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, United StatesAnna Malkova - Department of Biology, University of Iowa, Iowa City, IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Current opinion in genetics & development, Vol.71, pp.163-170
- DOI
- 10.1016/j.gde.2021.08.002
- PMID
- 34481360
- PMCID
- PMC9109633
- NLM abbreviation
- Curr Opin Genet Dev
- ISSN
- 0959-437X
- eISSN
- 1879-0380
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01 CA187052, R01 CA232425, R01 CA244912, R35 GM127006, R35 GM141868, R21 ES030307; DOI: 10.13039/100000054, name: NCI, award: R01 CA187052, R01 CA232425, R01 CA244912, R35 GM127006, R35 GM141868, R21 ES030307; DOI: 10.13039/100000057, name: NIGMS, award: R01 CA187052, R01 CA232425, R01 CA244912, R35 GM127006, R35 GM141868, R21 ES030307; DOI: 10.13039/100000066, name: NIEHS, award: R01 CA187052, R01 CA232425, R01 CA244912, R35 GM127006, R35 GM141868, R21 ES030307
- Language
- English
- Date published
- 12/2021
- Academic Unit
- Biology
- Record Identifier
- 9984217416702771
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