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Break-induced replication: unraveling each step
Journal article   Peer reviewed

Break-induced replication: unraveling each step

Liping Liu and Anna Malkova
Trends in genetics, Vol.38(7), pp.752-765
04/19/2022
DOI: 10.1016/j.tig.2022.03.011
PMCID: PMC9197877
PMID: 35459559
url
https://www.ncbi.nlm.nih.gov/pmc/articles/9197877View
Open Access

Abstract

Break-induced replication (BIR) repairs one-ended double-strand DNA breaks through invasion into a homologous template followed by DNA synthesis. Different from S-phase replication, BIR copies the template DNA in a migrating displacement loop (D-loop) and results in conservative inheritance of newly synthesized DNA. This unusual mode of DNA synthesis makes BIR a source of various genetic instabilities like those associated with cancer in humans. This review focuses on recent progress in delineating the mechanism of Rad51-dependent BIR in budding yeast. In addition, we discuss new data that describe changes in BIR efficiency and fidelity on encountering replication obstacles as well as the implications of these findings for BIR-dependent processes such as telomere maintenance and the repair of collapsed replication forks.
break-induced replication (BIR) lagging-strand BIR synthesis kinetics and rate of BIR yeast replication obstacles

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