Breast Milk CD4+ T Cells Express High Levels of C Chemokine Receptor 5 and CXC Chemokine Receptor 4 and Are Preserved in HIV-Infected Mothers Receiving Highly Active Antiretroviral Therapy

Athena P. Kourtis; Chris C. Ibegbu; Regan Theiler; Yong-Xian Xu; Pooja Bansil; Denise J. Jamieson; Michael Lindsay; Salvatore Butera; Ann Duer

doi:10.1086/512082

Back

Breast Milk CD4+ T Cells Express High Levels of C Chemokine Receptor 5 and CXC Chemokine Receptor 4 and Are Preserved in HIV-Infected Mothers Receiving Highly Active Antiretroviral Therapy

Journal article

Open access

Peer reviewed

Breast Milk CD4+ T Cells Express High Levels of C Chemokine Receptor 5 and CXC Chemokine Receptor 4 and Are Preserved in HIV-Infected Mothers Receiving Highly Active Antiretroviral Therapy

Athena P. Kourtis, Chris C. Ibegbu, Regan Theiler, Yong-Xian Xu, Pooja Bansil, Denise J. Jamieson, Michael Lindsay, Salvatore Butera and Ann Duer

The Journal of infectious diseases, Vol.195(7), pp.965-972

04/01/2007

DOI: 10.1086/512082

PMID: 17330786

Files and links (1)

url

https://doi.org/10.1086/512082View

Published (Version of record) Open Access

Abstract

Background. Transmission of human immunodeficiency virus (HIV) to the infant through breast-feeding is a major problem worldwide; however, the biological circumstances of such transmission remain unclear. Little characterization of breast milk CD4+ T lymphocytes has been done so far. Methods. We performed a detailed immunophenotypic analysis of T lymphocytes in the breast milk, compared with the blood, of HIV-uninfected (n = 9) and HIV-infected (n = 10) women receiving highly active antiretroviral therapy, by use of multiparameter flow cytometry. Descriptive statistics and nonparametric comparisons were performed using SAS software (version 9.1; SAS Institute). Results. In uninfected women, 44%–78% of breast milk CD4+ T cells expressed the C chemokine receptor 5 (CCR5), whereas 26%–73% of cells coexpressed CCR5 and CXC chemokine receptor 4 (CXCR4). In contrast, only 7%–20% of peripheral blood CD4+ T cells expressed CCR5 and 1%–20% coexpressed CCR5 and CXCR4. The level of CCR5 expression in CD4+ T cells in breast milk was higher than in blood. In HIV-infected women, the high frequency of CD4+CCR5+ T cells in breast milk was preserved. Conclusions. A majority of CD4+ T cells in breast milk express high levels of CCR5 and CXCR4. Unlike other mucosal immune sites, in which CD4+CCR5+ T cells are rapidly eliminated by HIV, these cells are preserved in breast milk during HIV infection.

Details

Title: Subtitle: Breast Milk CD4+ T Cells Express High Levels of C Chemokine Receptor 5 and CXC Chemokine Receptor 4 and Are Preserved in HIV-Infected Mothers Receiving Highly Active Antiretroviral Therapy
Creators: Athena P. Kourtis - Eastern Virginia Medical School
Chris C. Ibegbu - Emory University
Regan Theiler - Emory University
Yong-Xian Xu - Emory University School of Medicine
Pooja Bansil - National Center for Chronic Disease Prevention and Health Promotion
Denise J. Jamieson - Emory University
Michael Lindsay - Emory University
Salvatore Butera - Emory University
Ann Duer - Emory University School of Medicine
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.195(7), pp.965-972
DOI: 10.1086/512082
PMID: 17330786
NLM abbreviation: J Infect Dis
ISSN: 0022-1899
eISSN: 1537-6613
Publisher: The University of Chicago Press
Language: English
Date published: 04/01/2007
Academic Unit: Obstetrics and Gynecology; VPMA - Administration
Record Identifier: 9984446556802771

Metrics

17 Record Views

26 Times Cited - Web of Science