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Breast cancer patients from the Midwest region of the United States have reduced levels of short-chain fatty acid-producing gut bacteria
Journal article   Open access   Peer reviewed

Breast cancer patients from the Midwest region of the United States have reduced levels of short-chain fatty acid-producing gut bacteria

Rachel L Shrode, Jessica E Knobbe, Nicole Cady, Meeta Yadav, Jemmie Hoang, Catherine Cherwin, Melissa Curry, Rohan Garje, Praveen Vikas, Sonia Sugg, …
Scientific reports, Vol.13(1), pp.526-526
01/11/2023
DOI: 10.1038/s41598-023-27436-3
PMCID: PMC9834383
PMID: 36631533
url
https://doi.org/10.1038/s41598-023-27436-3View
Published (Version of record) Open Access

Abstract

As geographical location can impact the gut microbiome, it is important to study region-specific microbiome signatures of various diseases. Therefore, we profiled the gut microbiome of breast cancer (BC) patients of the Midwestern region of the United States. The bacterial component of the gut microbiome was profiled utilizing 16S ribosomal RNA sequencing. Additionally, a gene pathway analysis was performed to assess the functional capabilities of the bacterial microbiome. Alpha diversity was not significantly different between BC and healthy controls (HC), however beta diversity revealed distinct clustering between the two groups at the species and genera level. Wilcoxon Rank Sum test revealed modulation of several gut bacteria in BC specifically reduced abundance of those linked with beneficial effects such as Faecalibacterium prausnitzii. Machine learning analysis confirmed the significance of several of the modulated bacteria found by the univariate analysis. The functional analysis showed a decreased abundance of SCFA (propionate) production in BC compared to HC. In conclusion, we observed gut dysbiosis in BC with the depletion of SCFA-producing gut bacteria suggesting their role in the pathobiology of breast cancer. Mechanistic understanding of gut bacterial dysbiosis in breast cancer could lead to refined prevention and treatment.
Bacteria - genetics Breast Neoplasms Dysbiosis - microbiology Fatty Acids, Volatile Feces - microbiology Female Gastrointestinal Microbiome - genetics Humans RNA, Ribosomal, 16S - analysis RNA, Ribosomal, 16S - genetics United States - epidemiology

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