Journal article
Bro1 family proteins harmonize cargo sorting with vesicle formation
BioEssays, Vol.44(8), pp.e2100276-n/a
08/2022
DOI: 10.1002/bies.202100276
PMCID: PMC9575758
PMID: 35770783
Abstract
The Endosomal Sorting Complexes Required for Transport (ESCRTs) drive membrane remodeling in a variety of cellular processes that include the formation of endosomal intralumenal vesicles (ILVs) during multivesicular body (MVB) biogenesis. During MVB sorting, ESCRTs recognize ubiquitin (Ub) attached to membrane protein cargo and execute ILV formation by controlling the activities of ESCRT‐III polymers regulated by the AAA‐ATPase Vps4. Exactly how these events are coordinated to ensure proper cargo loading into ILVs remains unclear. Here we discuss recent work documenting the ability of Bro1, an ESCRT‐associated Ub‐binding protein, to coordinate ESCRT‐III and Vps4‐dependent ILV biogenesis with upstream events such as cargo recognition.
Model of Bro1‐mediated stimulation of Vps4 and intralumenal vesicle (ILV) formation. Bro1 V domain binds and stimulates Vps4 to drive ILV formation, and regulation of this stimulation is required for coordinating cargo sorting. Ubiquitin binding to the V domain can further enhance this mode of stimulation. These ideas build upon previous understandings of Bro1 interactions (ESCRT‐III, Ubiquitin, YPxL, ESCRT‐I, DUbs, and Ub ligase) and outcomes (cargo recognition). BOD, Bro1 domain; DUbs, deubiquitinases; PRR, proline rich domain; ILV, intralumenal vesicle; Ub, ubiquitin; YPxL, amino acids tryptophan, proline, [x], leucine.
Details
- Title: Subtitle
- Bro1 family proteins harmonize cargo sorting with vesicle formation
- Creators
- Chun‐Che Tseng - Mayo Clinic Graduate School of Biomedical Sciences, Mayo ClinicRobert C. Piper - Roy J. and Lucille A. Carver College of MedicineDavid J. Katzmann - Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA.
- Resource Type
- Journal article
- Publication Details
- BioEssays, Vol.44(8), pp.e2100276-n/a
- DOI
- 10.1002/bies.202100276
- PMID
- 35770783
- PMCID
- PMC9575758
- NLM abbreviation
- Bioessays
- ISSN
- 0265-9247
- eISSN
- 1521-1878
- Number of pages
- 8
- Grant note
- National Institutes of Health (R01 GM116826; R01 GM58202)
- Language
- English
- Date published
- 08/2022
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297603702771
Metrics
67 Record Views